找不到产品？留下需求帮您找
一键询价
-
瑞士万通Multi Autolab/M204多通道电化学工作站型号： Multi Autolab/M204 -
德国FRITSCH P7 小型行星式球磨机/仪加强型型号： PULVERISETTE 7 premium line -
激光干涉仪型号： XL80 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
Hitachi日立超速离心机CP70ME型号： CP70ME -
电热鼓风干燥箱型号： CS101-ABN -
Agilent 8890 气相色谱系统型号： 8890 GC -
T700/T600 系列紫外可见分光光度计型号： T700/T600 -
日立Primaide PLUS高效液相色谱仪型号： Primaide PLUS
获取电话
Klotho is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. Human Klotho exists in both-membrane bound and secreted forms, and is predominantly expressed in the kidney convoluted tubules, and, to a lesser extent, in the brain, reproductive organs, endocrine glands, urinary bladder, skeletal muscle, placenta, and colon. The full-length transmembrane form has a large extracellular domain composed of two homologous subunits termed KL1 and KL2, which contain 516 and 439 amino acid residues, respectively. The predominant circulating form, which is derived from alternative RNA splicing, contains the KL1 subunit and constitutes the N-terminal sequence of transmembrane Klotho. A third Klotho protein of about 128 kDa has been identified in the blood and cerebrospinal fluid. This circulating protein arises from the action of an as yet unidentified protease, which cleaves transmembrane Klotho just above and/or within the plasma membrane. Klotho has been shown to play a key role in the signaling cascade of fibroblast growth factor-23 (FGF-23), a bone-derived hormone that acts in the kidney to inhibit phosphate reabsorption and vitamin D biosynthesis. Klotho promotes FGF-23 signaling through binding to FGFRI (IIIc) which converts this canonical FGF receptor into a specific receptor for FGF-23. In the absence of Klotho the function of FGF-23 is literally abolished. Recombinant Human Klotho is a glycoprotein of 516 amino acid residues that migrates at an apparent molecular weight of 65-70 kDa by SDS-PAGE analysis under reducing conditions. Recombinant Human Klotho has a calculated molecular weight of 58.6 kDa.
Source: CHO cells
Synonyms: αKlotho, KLA
AA Sequence: EPGDGAQTWA RFSRPPAPEA AGLFQGTFPD GFLWAVGSAA YQTEGGWQQH GKGASIWDTF THHPLAPPGD SRNASLPLGA PSPLQPATGD VASDSYNNVF RDTEALRELG VTHYRFSISW ARVLPNGSAG VPNREGLRYY RRLLERLREL GVQPVVTLYH WDLPQRLQDA YGGWANRALA DHFRDYAELC FRHFGGQVKY WITIDNPYVV AWHGYATGRL APGIRGSPRL GYLVAHNLLL AHAKVWHLYN TSFRPTQGGQ VSIALSSHWI NPRRMTDHSI KECQKSLDFV LGWFAKPVFI DGDYPESMKN NLSSILPDFT ESEKKFIKGT ADFFALCFGP TLSFQLLDPH MKFRQLESPN LRQLLSWIDL EFNHPQIFIV ENGWFVSGTT KRDDAKYMYY LKKFIMETLK AIKLDGVDVI GYTAWSLMDG FEWHRGYSIR RGLFYVDFLS QDKMLLPKSS ALFYQKLIEK NGFPPLPENQ PLEGTFPCDF AWGVVDNYIQ VSQLTKPISS LTKPYH
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of murine NIH-3T3 cells. Recombinant Human Klotho is effective in a concentration range of 0.5-2.0 μg/ml.
Recombinant Human Klotho Biological Activity Graph
Calculated Molecular Weight: 58.6 kDa
Accession Number: Q9UEF7
Gene ID: 9365
Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug)
Note:
1mg will be provided as 2x500μg.
Cross Reactivity Cited in References:
Human, Mouse, Rat
