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- 保存条件:-20℃
溶菌酶 (≥40KU/mg, 进口)
Lysozyme from chicken egg white
产品规格: 1g
CAS: 12650-88-3
保存条件: -20℃保存
产品介绍：
CAS号：12650-88-3
分子量：single-chain 14.3kDa
MDL：MFCD00131557
级别/活性：BR,≥40,000 units/mg protein
纯度：≥90% (HPLC)
外观/性状：lyophilized powder
酶活性定义：One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).
chem/physiol Actions:
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
L6876 (Sigma-Aldrich)
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