获取电话别称
NIFUN
宿主
Rabbit
适应种属
Human, Mouse, Rat
亚型
IgG
验证应用
ELISA, WB, IHC, IF, IP
产品信息
产品名称
anti- ISCU antibody
货号
QSab04402
规格
100μg
状态
liquid
纯化方法
Immunogen affinity purified
纯度
≥95% as determined by SDS-PAGE
宿主
Rabbit
克隆性
polyclonal
亚型
IgG
存储
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
背景介绍
Scaffold protein for the de novo synthesis of iron-sulfur (Fe-S) clusters within mitochondria, which is required for maturation of both mitochondrial and cytoplasmic [2Fe-2S] and [4Fe-4S] proteins (PubMed:11060020) . First, a [2Fe-2S] cluster is transiently assembled on the scaffold protein ISCU. In a second step, the cluster is released from ISCU, transferred to a glutaredoxin GLRX5, followed by the formation of mitochondrial [2Fe-2S] proteins, the synthesis of [4Fe-4S] clusters and their target-specific insertion into the recipient apoproteins. Cluster assembly on ISCU depends on the function of the cysteine desulfurase complex NFS1-LYRM4/ISD11, which serves as the sulfur donor for cluster synthesis, the iron-binding protein frataxin as the putative iron donor, and the electron transfer chain comprised of ferredoxin reductase and ferredoxin, which receive their electrons from NADH (By similarity) .
免疫原信息
免疫原
iron-sulfur cluster scaffold homolog (E. coli)
别称
NIFUN
分子量
14-15 kDa, 18-19 kDa
应用
验证应用
ELISA, WB, IHC, IF, IP
建议稀释比例
WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200; IF: 1:20-1:200