获取电话Anti-APOER2 antibody
种属反应性Human
验证应用WB,ICC
抗体类型小鼠单抗
免疫原Recombinant protein corresponding to N terminal of Human APOER2.
偶联Non-conjugated
Anti-APOER2 antibody性能
形态Liquid
浓度2 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2a
纯化方式Protein A purified
亚细胞定位Cell membrane, Membrane, Secreted.
其它名称
moreAPOER2 antibody
Apolipoprotein E receptor 2 antibody
low density lipoprotein receptor-related protein 8 antibody
Anti-APOER2 antibody应用
WB: 1:500
ICC: 1:50
Fig1: Western blot analysis of APOER2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: SHSY5Y cell lysate
Lane 3: SKBR-3 cell lysate
Lane 4: U937 cell lysate
Fig2: ICC staining of APOER2 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
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