获取电话Anti-FAM62B antibody
种属反应性Human
验证应用WB,IHC
抗体类型小鼠单抗
免疫原Recombinant protein within human FAM62B aa 300-550.
偶联Non-conjugated
Anti-FAM62B antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein G affinity purified.
亚细胞定位Cell membrane, Endoplasmic reticulum, Membrane.
其它名称
moreChr2 synaptotagmin antibody
CHR2SYT antibody
E Syt2 antibody
Anti-FAM62B antibody应用
WB: 1:500
IHC: 1:50-1:200
Fig1: Western blot analysis of FAM62B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Siha cell lysate
Lane 2: THP-1 cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FAM62B antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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