找不到产品？留下需求帮您找
一键询价
-
德国耶拿 multi N/C 3100 TOC总有机碳/总氮分析仪型号： multi N/C 3100 -
日立ZA4000系列原子吸收分光光度计型号： ZA4000 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
舜宇恒平 GC1290/MS8100 气相色谱-质谱联用仪型号： GC1290/MS8100 -
电热鼓风干燥箱型号： CS101-ABN -
Agilent GC 8890 气相色谱系统型号： 8890 GC -
日本Rigaku智能多功能X射线衍射仪Smartlab SE型号： Smartlab SE -
Ugo Basile动态足底触觉仪,足底触觉测试仪型号： Ugo Basile动态足底触觉仪,足底触觉测试仪 -
上海辰华电化学工作站CHI760E型号： CHI760E
获取电话Anti-CD68 antibody
种属反应性Human
验证应用WB,IHC-P,FC
抗体类型小鼠单抗
免疫原Synthetic peptide within human CD68 aa 320-354.
偶联Non-conjugated
Anti-CD68 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein G affinity purified.
亚细胞定位Cell membrane. Endosome membrane, lysosome membrane.
其它名称
moreCD 68 antibody
CD68 antibody
CD68 antigen antibody
Anti-CD68 antibody应用
WB:1:1,000-1:2,000
IHC-P:1:50-1:200
FC:1:50-1:100
Fig1: Western blot analysis of CD68 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: THP-1 cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-CD68 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig5: Flow cytometric analysis of CD68 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody(red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
Anti-CD68 antibody多少钱?
泽叶生物抗体/抗原的应用资料有吗？
泽叶生物抗体/抗原货号ZY6901-95M 报价含票含运吗？
泽叶生物货号ZY6901-95M 货期是多久？
泽叶生物Anti-CD68 antibody可以申请试用吗？
泽叶生物泽叶生物抗体/抗原售后政策是什么？
Anti-CD68 antibody有现货吗？
