获取电话Anti-HLA-B antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Recombinant protein
偶联Non-conjugated
Anti-HLA-B antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Membrane
其它名称
moreAS antibody
HLA B protein antibody
HLA class I histocompatibility antigen, B alpha chain antibody
Anti-HLA-B antibody应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:200
Fig1: Western blot analysis of HLA-B on human HLA-B recombinant protein using anti-HLA-B antibody at 1/1,000 dilution.
Fig2: Western blot analysis of HLA-B on HEK293 (1) and HLA-B-hIgGFc transfected HEK293 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
Fig3: Western blot analysis of HLA-B on Ramos(1) and A431 (2) cell lysate using anti-HLA-B antibody at 1/1,000 dilution.
Fig4: ICC staining HLA-B (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-HLA-B antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of MCF-7 cells with HLA-B antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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