获取电话Anti-Bmi1 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Recombinant protein within human Bmi1 full sequence.
偶联Non-conjugated
Anti-Bmi1 antibody性能
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG2a
纯化方式Protein A purified.
亚细胞定位Nucleus, Cytoplasm
其它名称
moreB lymphoma Mo MLV insertion region (mouse) antibody
B lymphoma Mo MLV insertion region 1 homolog antibody
Bmi 1 antibody
Anti-Bmi1 antibody应用
WB: 1:1,000
IHC-P: 1:200
ICC: 1:200
FC: 1:100-1:200
Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: 293T
Lane 2: Jurkat
Lane 3: Hela
Lane 4: MCF-7
Lane 5: HepG2
Lane 6: NIH/3T3
Lane 7: PC12
Lane 8: Mouse kidney
Lane 9: Human kidney
Lane 10: K562
Lane 11: Human brain
Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
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Anti-Bmi1 antibody多少钱?
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