获取电话Anti-Beta-galactosidase antibody
种属反应性Human
验证应用WB,IHC
抗体类型兔多抗
免疫原Synthetic peptide within human Beta-galactosidase aa 610-677.
Anti-Beta-galactosidase antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cytoplasm, Lysosome.
其它名称
moreAcid beta galactosidase antibody
Acid beta-galactosidase antibody
Beta galactosidase 1 antibody
Anti-Beta-galactosidase antibody应用
WB: 1:1,000-1:2,000
IHC: 1:100-1:500
Fig1: Western blot analysis of Beta-galactosidase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-galactosidase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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