获取电话Anti-Smoothelin antibody
种属反应性Human
验证应用WB,IHC
抗体类型兔多抗
免疫原Recombinant protein within human Smoothelin aa 300-487.
偶联Non-conjugated
Anti-Smoothelin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Cytoplasm, Cytoskeleton.
其它名称
moreFLJ35168 antibody
FLJ35365 antibody
FLJ35620 antibody
Anti-Smoothelin antibody应用
WB: 1:500-1:1,000
IHC: 1:50
Fig1: Western blot analysis of Smoothelin on PC-3M cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunohistochemical analysis of paraffin-embedded human uterine fibroids tissue using anti-Smoothelin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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