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Anti-Dux antibody

Anti-Dux antibody

价格: 1380

品牌:泽叶生物

供货周期: 现货

货号:ZY6001-60R

型号:兔多抗

Anti-Dux antibody


  • 种属反应性Mouse

  • 验证应用WB,ICC

  • 抗体类型兔多抗

  • 免疫原Synthetic peptide within mouse Dux aa 520-570.

  • 偶联Non-conjugated

  • Anti-Dux antibody性能

  • 形态Liquid

  • 浓度1 mg/ml.

  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

  • 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • 亚型IgG

  • 纯化方式Peptide affinity purified.

  • 亚细胞定位Nucleus.

  • 其它名称

    more
    • Double homeobox 4 antibody

    • Double homeobox protein 10 antibody

    • Double homeobox protein 4 antibody

  • Anti-Dux antibody应用

    WB: 1:500
    ICC: 1:50-1:200

  • Fig1: Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Non-transfected 293T cells lysate
    Lane 2: His-tagged Dux transfected 293T cells lysate

    Fig2: ICC staining of Dux in Non-transfected 293T and His-tagged Dux transfected 293T cells cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

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生化试剂 Anti-Dux antibody由上海泽叶生物科技有限公司为您提供,如想了解更多关于抗体/抗原 Anti-Dux antibody的报价、规格、厂家等信息,欢迎来电或留言咨询。除供应Anti-Dux antibody外,上海泽叶生物科技有限公司还可为您提供: 彩色预染蛋白Marker(10-180 kDa,三色)、 Super GelBlueTM 核酸染料, 10,000× in water 、 彩色预染蛋白Marker(10-180 kDa,三色)

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