获取电话Anti-Flavin-containing monooxygenase antibody
种属反应性Rice
验证应用WB,IF,IHC-P
抗体类型兔多抗
免疫原Synthetic peptide within rice Flavin-containing monooxygenase aa 1-30.
偶联Non-conjugated
Anti-Flavin-containing monooxygenase antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Integral component of membrane.
其它名称
Flavin-containing monooxygenase
OsI_14691
Anti-Flavin-containing monooxygenase antibody应用
WB:1:500-1:2,000
IF:1:50-1:100
IHC-P:1:50-1:200
Fig1: Western blot analysis of Flavin-containing monooxygenase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunofluorescence staining of paraffin- embedded rice tissue using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
Fig3: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Flavin-containing monooxygenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Anti-Flavin-containing monooxygenase antibody多少钱?
泽叶生物抗体/抗原的应用资料有吗？
泽叶生物抗体/抗原货号ZY6902-44R报价含票含运吗？
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