获取电话Anti-Afadin/I Afadin antibody
种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human AF6 341-440/1816
偶联Non-conjugated
Anti-Afadin/I Afadin antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell junction, adherens junction. Note=Not found at cell-matrix AJs.
其它名称
moreAnti-Afadin/I Afadin antibody应用
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-Afadin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kD
Observed band size: 135/138 kD
Fig2: Sample:
Skin (Rat) Lysate at 40 ug
Primary: Anti-Afadint 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kD
Observed band size: 135/138 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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