ATCC 30166 胎儿三毛滴虫 百欧博伟生物

       ATCC 30166 胎儿三毛滴虫 百欧博伟生物

胎儿三毛滴虫是一种寄生在结肠的原虫，形态与贾第鞭毛虫相似，贾第鞭毛虫引起的是小肠性腹泻，而胎儿三毛滴虫引起的是大肠性腹泻，二者也可以合并感染。

基本信息

平台编号：bio-48651

规格：frozen

拉丁属名：Tritrichomonas foetus

中文名称：胎儿三毛滴虫

详情介绍

Tritrichomonas foetus (Riedmuller) Wenrich and Emerson 拉丁名

(ATCC? 30166?) 统一编号

Strain Designations 菌种别名 Belfast

Biosafety Level 生物安全等级 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation 分离源 Bos bovis, Belfast, Ireland, 1938

Product Format 提供形式 frozen

Storage Conditions 保存方法

Frozen Cultures 冷冻物 : -70°C for 1 week; liquid N2 vapor for long term storage

Freeze-dried Cultures 冻干物 : 2-8°C

Live Cultures 活菌 : See Protocols section for handling information

Type Strain 模式菌株 no

Comments Geographic variation

Medium 培养基 ATCC? Medium 2154: LYI Entamoeba medium

ATCC? Medium 359: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 7.2 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use

Growth Conditions 生长条件

Temperature 培养温度 : 35°C

Culture System: Axenic

Subcultivation Protocol: at pH 7.0

Cryopreservation Harvest and Preservation Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min.

When cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant.

Adjust the concentration of cells to 2 x 106 - 2 x 107/mL in fresh medium. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium.

Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube; Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium; Invert several times to dissolve the DMSO; Allow to warm to room temperature. Mix the cell preparation and the DMSO in equal portions.

Thus, the final concentration will be 106 - 107 cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min.

Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).

Place the vials in a controlled rate freezing unit.  

From room temperature cool at -1°C/min to -40°C.  If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion.

At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.  Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.(The cooling rate in this apparatus is approximately -1°C/min.)  

The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely.

Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C.

To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material.

Do not agitate the vial. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 359 (completed with serum) or 13 mL ATCC Medium 2154.

Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 359 or on a 15° horizontal slant for medium 2154). Mycoplasma Unknown

Name of Depositor 寄存人 R Samuels

Special Collection NSF - Protistology

Chain of Custody 来源国家 ATCC <-- R Samuels <-- M. Robertson <-- W.R. Kerr

Year of Origin 1938

References 参考文献 J. Hyg. 58: 207-213, 1960. Torian BE, et al. Specific and common antigens of Trichomonas vaginalis detected by monoclonal antibodies. Infect. Immun. 43: 270-275, 1984. PubMed: 6360900

Krieger JN, et al. Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. J. Infect. Dis. 152: 979-984, 1985. PubMed: 2413147

Felleisen RS, et al. Detection of Tritrichomonas foetus by PCR and DNA enzyme immunoassay based on rRNA gene unit sequences. J. Clin. Microbiol. 36: 513-519, 1998. PubMed: 9466768

Felleisen RS. Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique. Parasitol. Res. 84: 153-156, 1998. PubMed: 9493217

Felleisen RS. Comparative sequence analysis of 5.8S rRNA genes and internal transcribed spacer (ITS) regions of trichomonadid protozoa. Parasitology 115: 111-119, 1997. PubMed: 10190167

Bouma MJ, et al. Activity of disulfiram (bis(diethylthiocarbamoyl)disulphide) and ditiocarb (diethyldithiocarbamate) against metronidazole-sensitive and -resistant Trichomonas vaginalis and Tritrichomonas foetus. J. Antimicrob. Chemother. 42: 817-820, 1998. PubMed: 10052908

Babal P, et al. Sialic acid-specific lectin from Tritrichomonas foetus. Biochim. Biophys. Acta 1428: 106-116, 1999. PubMed: 10366765

Wei-Dong Xu, et al. Chromosome numbers of Tritrichomonas foetus and Tritrichomonas suis. Vet. Parasitol. 78: 247-251, 1998. PubMed: 9786624

Lun Z-R, et al. Comparison of growth rates of Tritrichomonas foetus isolates from various geographic regions using three different culture media. Vet. Parasitol. 89: 199-208, 2000. PubMed: 10760410

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