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产品描述： 产品名称：Recombinant Equine IL-1β 氨基酸序列: AAMHSVNCRL RDIYHKSLVL SGACELQAVH LNGENTNQQV VFCMSFVQGE EETDKIPVAL GLKEKNLYLS CGMKDGKPTL QLETVDPNTY PKRKMEKRFV FNKMEIKGNV EFESAMYPNW YISTSQAEKS PVFLGNTRGG RDITDFIMEI TSA 参考文献: 1. Bankers-Fulbright, J.L., K.R. Kalli, and D.J. McKean. 1996. Life Sci, 59: 61-83.<br/>2. Dinarello, C.A. 1997. Semin Oncol, 24: S9-81-S9-93.<br/>3. Martinon, F., and J. Tschopp. 2007. Cell Death Differ, 14: 10-22.<br/>4. Auron, P.E., A.C. Webb, L.J. Rosenwasser, et al. 1984. Proc Natl Acad Sci U S A, 81: 7907-11. Accession: <a href="http://www.uniprot.org/uniprot/Q28386" target="_blank">Q28386</a> Gene IDs: <a href="http://www.ncbi.nlm.nih.gov/gene/100034237" target="_blank">100034237</a> 种属: Equine 纯度: > 95 % by SDS-PAGE and HPLC analyses. 来源: Escherichia coli. 内毒素水平: Less than 1 EU/μg of rEqIL-1β as determined by LAL method. 活性: Fully biologically active when compared to standard. The ED<sub>50</sub> as determined by a cell proliferation assay using murine D10S cells is less than 20 pg/ml, corresponding to a specific activity of > 5.0 × 10<sup>7</sup> IU/mg. 形态: Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, 0.1 % Tween-80. 溶解方法: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. 储存/保存方法: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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