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微管蛋白β tubulin/Tubulin β抗体
英文名称 Beta tubulin
中文名称 微管蛋白β tubulin/Tubulin β抗体
别 名 Beta 4 tubulin; Tubulin-beta; Tubulin beta; Beta 5 tubulin; BetaTubulin; Beta-Tubulin; dJ40E16.7; TUBB; TUBB2; TUBB2A; TUBB5; tubulin beta 2A; Tubulin beta chain; Tubulin beta-5 chain; TUBB4A; TUBB4; Tubulin 5 beta; Tubulin beta-4 chain; TBB4A_HUMAN; Tubulin beta-4A chain.
研究领域 细胞生物 免疫学 神经生物学 细胞骨架
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Rabbit,
产品应用 WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 55kDa
细胞定位 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Beta tubulin:61-160/444
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.
结构蛋白（Structural Proteins）
tubulin是一种大量存在于哺乳动物脑组织中的微管亚基蛋白，在结构上是由两个极为相近的α和β亚基组成的二聚体、多聚体形成微管细丝，是微管的主要成分。
微管蛋白是球形分子, 有两种类型:α微管蛋白(α-tubulin)货号：bs-0195R和β微管蛋白(β-tubulin), 这两种微管蛋白具有相似的三维结构, 能够紧密地结合成二聚体, 作为微管组装的亚基。 α亚基由450个氨基酸组成, β亚基是由455个氨基酸组成, 这两种亚基有35～40%的氨基酸序列同源, 表明编码它们的基因可能是由同一原始祖先演变而来.
