获取电话粘蛋白3(MUC3)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Secreted, Membrane; Single-pass membrane prote
预测分子量43.7kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Cys2176~Ala2280 with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 95%
等电点5.4
粘蛋白3(MUC3)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Mucin 3 (MUC3)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Cys2176~Ala2280
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: Q02505
Host: E. coli
Subcellular Location: Secreted, Membrane;
Single-pass membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.4
Predicted Molecular Mass: 43.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
粘蛋白3(MUC3)重组蛋白[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
粘蛋白3(MUC3)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
CDNGG TWEQGQCACL PGFSGDRCQL QTRCQNGGQW DGLKCQCPST FYGSSCEFAV
EQVDLDVVET EVGMEVSVDQ QFSPDLNDNT SQAYRDFNKT FWNQMQKIFA
[ REFERENCES ]
1. Hillier L.W., et al. (2003) Nature 424:157-164. 2. Gum J.R. Jr., et al. (2003) J. Biol. Chem. 278:49600-49609. 3. Pratt W.S., et al. (2000) Biochem. Biophys. Res. Commun. 275:916-923. 4. Kyo K., et al. (2001) J. Hum. Genet. 46:5-20.
