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激光干涉仪型号： XL80 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
Hitachi日立超速离心机CP70ME型号： CP70ME -
电热鼓风干燥箱型号： CS101-ABN -
Agilent 8890 气相色谱系统型号： 8890 GC -
全自动桌面溶出度测试系统RTJ3000+DS-3000型号： RT-J3000+DS3000 -
全自动溶出度测试仪RT-3型号： RT-3 -
流通池法溶出度测试仪DF-7型号： DF-7 -
自动桌面溶出度测试系统RT-J2000+DS2000α/DS3000型号： RT-J2000+DS2000α/DS3000 -
日立Primaide PLUS高效液相色谱仪型号： Primaide PLUS
获取电话乳铁传递蛋白(LTF)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Secreted. Cytoplasmic granule.
预测分子量40.3kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Val25~Glu352 (Accession # P24627) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 95%
等电点9.2
乳铁传递蛋白(LTF)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Lactoferrin (LTF)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Val25~Glu352 (Accession # P24627),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Secreted. Cytoplasmic
granule.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.2
Predicted Molecular Mass: 40.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
乳铁传递蛋白(LTF)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
乳铁传递蛋白(LTF)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS - VRWCTI SQPEWFKCRR
WQWRMKKLGA PSITCVRRAF ALECIRAIAE KKADAVTLDG GMVFEAGRDP YKLRPVAAEI
YGTKESPQTH YYAVAVVKKG SNFQLDQLQG RKSCHTGLGR SAGWIIPMGI LRPYLSWTES
LEPLQGAVAK FFSASCVPCI DRQAYPNLCQ LCKGEGENQC ACSSREPYFG YSGAFKCLQD
GAGDVAFVKE TTVFENLPEK ADRDQYELLC LNNSRAPVDA FKECHLAQVP SHAVVARSVD
GKEDLIWKLL SKAQEKFGKN KSRSFQLFGS PPGQRDLLFK DSALGFLRIP SKVDSALYLG
SRYLTTLKNL RE
