找不到产品？留下需求帮您找
一键询价
-
德国耶拿 multi N/C 3100 TOC总有机碳/总氮分析仪型号： multi N/C 3100 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
电热鼓风干燥箱型号： CS101-ABN -
Agilent GC 8890 气相色谱系统型号： 8890 GC -
隔膜式压缩机型号： 隔膜式气体压缩机 -
布鲁克400MHz核磁共振NMR波谱仪 AVANCE NEO 400M型号： AVANCE NEO 400M -
T700/T600 系列紫外可见分光光度计型号： T700/T600 -
天美公司赛里安456i气相色谱仪型号： 456i -
珀金埃尔默PerkinElmer NexION 1100/1000G ICP-MS型号： NexION 1100/1000G ICP-MS
获取电话颗粒酶A(GZMA)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Secreted; Cytoplasmic granu
预测分子量27.2kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Ile29~Val260 (Accession # P11032) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 97%
等电点9.4
颗粒酶A(GZMA)重组蛋应用SDS-PAGE; WB; ELISA; IP
Recombinant Granzyme A (GZMA)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Ile29~Val260 (Accession # P11032), with
N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted; Cytoplasmic
granule. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.4
Predicted Molecular Mass: 27.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
颗粒酶A(GZMA)重组蛋[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
颗粒酶A(GZMA)重组蛋[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-II GGDTVVPHSR PYMALLKLSS NTICAGALIE KNWVLTAAHC
NVGKRSKFIL GAHSINKEPE QQILTVKKAF PYPCYDEYTR EGDLQLVRLK KKATVNRNVA
ILHLPKKGDD VKPGTRCRVA GWGRFGNKSA PSETLREVNI TVIDRKICND EKHYNFHPVI
GLNMICAGDL RGGKDSCNGD SGSPLLCDGI LRGITSFGGE KCGDRRWPGV YTFLSDKHLN
WIKKIMKGSV
[ REFERENCES ]
1. Ebnet K., et al. (1992) Genomics 13:502-508. 2. Hershberger R.J., et al. (1992) J. Biol. Chem. 267:25488-25493. 3. Jenne D.E., Tschopp J. (1988) Immunol. Rev. 103:53-71. 4. Simon H.G., et al. (1988) Eur. J. Immunol. 18:855-861.
