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激光干涉仪型号： XL80 -
Hitachi日立CT15RE台式离心机型号： CT15RE -
Hitachi日立超速离心机CP70ME型号： CP70ME -
电热鼓风干燥箱型号： CS101-ABN -
Agilent 8890 气相色谱系统型号： 8890 GC -
全自动桌面溶出度测试系统RTJ3000+DS-3000型号： RT-J3000+DS3000 -
全自动溶出度测试仪RT-3型号： RT-3 -
流通池法溶出度测试仪DF-7型号： DF-7 -
自动桌面溶出度测试系统RT-J2000+DS2000α/DS3000型号： RT-J2000+DS2000α/DS3000 -
日立Primaide PLUS高效液相色谱仪型号： Primaide PLUS
获取电话凝溶胶蛋白(GS)重组蛋白
来源原核表达
宿主E.coli
内毒素水平<1.0EU/μg(LAL法测定)
亚细胞定位Secreted
预测分子量13.2kDa
实际分子量-(差异分析请参阅说明书)
片段与标签Val20~Ala102 (Accession # Q2KIQ8) with N-terminal His Tag
缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
性状冻干粉
纯度> 95%
等电点10.5
凝溶胶蛋白(GS)重组蛋白应用SDS-PAGE; WB; ELISA; IP
Recombinant Interferon Gamma Induced Protein 10kDa (IP10)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PROPERTIES ]
Residues: Val20~Ala102
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q2KIQ8
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 10.5
Predicted Molecular Mass: 13.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
凝溶胶蛋白(GS)重组蛋白[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
凝溶胶蛋白(GS)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
V PLSRNTRCSC IEISNGSVNP RSLEKLEVIP ASQSCPRVEI IATMKKNGEK RCLNPESKTI
KNLLKAINKQ RTKRSPRTRK EA
[ REFERENCES ]
1. Hudgens T., et al. (2007) Submitted to the EMBL/GenBank/DDBJ databases. 2. Luster AD., et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84 (9): 2868–71.
3. Angiolillo AL., et al. (1995) J. Exp. Med. 182 (1): 155–62.
4. Booth V., et al. (2002) Biochemistry 41 (33): 10418–25.
