产品货号:ZZK-K-ARAB
产品品名:阿拉伯聚糖检测试剂盒
英文品名:Arabinan Assay Kit
规格型号:100 assays per kit
For the measurement of arabinan in fruit juice concentrates.
INTRODUCTION:
In the processing of apples and pears, the yield of juice can be
dramatically improved by using enzymes to degrade the pulp
polysaccharides, together with more exhaustive extraction of the
pulp with diffusion equipment. Of course, these processes also
significantly increase the amount of partially degraded polysaccharide
which is solubilised. This polysaccharide material may be soluble as
extracted, but subsequent changes in temperature and pH conditions
can directly lead to precipitation (or crystallisation) or to chemical
modification followed by precipitation. Such a problem can be
experienced in the production of clear apple or pear juice, in which
case, an arabinan haze material is produced. This material was shown
to be microcrystalline 1,5-α-L-arabinan.
Arabinans, as present in cell-wall pectic-substances, have been shown
to consist of a main chain of 1,5-α-linked L-arabinofuranosyl residues
to which other L-arabinofuranosyl residues are linked 1,3-α and 1,2-α
in either a comb-like or a ramified arrangement.
It is generally accepted that the best solution to this “arabinan
haze” problem is to use pectinase enzyme preparations containing
high levels of both α-L-arabinofuranosidase and endo-1,5-α-Larabinanase.
The combined action of these two enzymes reduces
arabinan to arabinose and lower degree of polymerisation, arabinooligosaccharides.
KITS:
Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02 %
w/v) as a preservative (Buffer A).
Stable for > 2 years at 4°C.
Bottle 2: NAD+.
Stable for > 5 years at -20°C.
Bottle 3: α-L-Arabinofuranosidase plus endo-arabinanase
suspension (2.2 mL).
Stable for > 4 years at 4°C.
Bottle 4: β-Galactose dehydrogenase plus galactose mutarotase
suspension, 2.2 mL.
Stable for > 2 years at 4°C.
Bottle 5: L-Arabinose standard solution (5 mL, 0.5 mg/mL in
0.02 % sodium azide).
Stable for > 4 years at 4°C.
Bottle 6: Arabinan control in mannitol: Arabinan
concentration on vial.
Stable for > 5 years at 4°C.
Component 7: Pharmacia PD-10 column.
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