磷酸化染色体结构维持蛋白质１抗体

英文名称    Phospho-SMC1(Ser957)    

中文名称    磷酸化染色体结构维持蛋白质１抗体    

别    名    SMC1(Phospho-Ser957); SMC1A(phospho S957); P-SMC1(Ser957);CDLS2; SMC1; Segregation of mitotic chromosomes 1; Segregation of mitotic chromosomes like 1; SMC 1; SMC1A; SMC1alpha; SMC1alpha protein; SMC1B; SMC1BETA; SMC1beta protein; SMC1L1; SMC1L2; SMCB; Structural maintenance of chromosome 1 like 1 protein; Structural maintenance of chromosome 1 like 2 protein; structural maintenance of chromosomes 1-like 2 (yeast); Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes 1B; Structural Maintenance of Chromosomes-1 Like 1; SMC1A_HUMAN.    

磷酸化染色体结构维持蛋白质１抗体        

说 明 书    0.1ml      

产品类型    磷酸化抗体     

研究领域    肿瘤  细胞生物  免疫学  染色质和核信号  转录调节因子      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    136kDa    

细胞定位    细胞核     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human SMC1 around the phosphorylation site of Ser957 [GS(p-S)QG]    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化染色体结构维持蛋白质１抗体 产品介绍    background:
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes and can lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion complex associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo.

Function:
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesion complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Subunit:
Interacts with POLE. Interacts with SYCP2. Interacts with BRCA1. Found in a complex with CDCA5, SMC3 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC3 in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. In germ cell cohesion complexes, SMC1A is mutually exclusive with SMC1B. Interacts with BRCA1. Interacts with NDC80.

Subcellular Location:
Nucleus. Chromosome. Chromosome, centromere, kinetochore. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesion complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesion complexes remain. At anaphase, the RAD21 subunit of the cohesion complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesion complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis

Post-translational modifications:
Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

DISEASE:
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.

Similarity:
Belongs to the SMC family. SMC1 subfamily.

Gene ID:
27127

Database links:

Entrez Gene: 27127 Human

Entrez Gene: 8243 Human

Entrez Gene: 140557 Mouse

Entrez Gene: 24061 Mouse

Entrez Gene: 300121 Rat

Entrez Gene: 63996 Rat

Omim: 300040 Human

Omim: 608685 Human

SwissProt: Q14683 Human

SwissProt: Q8NDV3 Human

SwissProt: Q920F6 Mouse

SwissProt: Q9CU62 Mouse

SwissProt: Q9Z1M9 Rat

Unigene: 334176 Human

Unigene: 182737 Mouse

Unigene: 56972 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

磷酸化染色体结构维持蛋白质１抗体 

hz-1534R LC3 α/MAP1A/MAP LC3 Alpha/Beta  自噬微管相关蛋白轻链3抗体

hz-5076R LASS1  长寿相关基因lASS1抗体

hz-5507R phospho-MAP1LC3A(Ser12)  磷酸化自噬微管相关蛋白轻链3抗体

hz-5077R LASS2  肿瘤转移抑制基因1抗体

hz-5078R LASS3  长寿相关基因lASS3抗体

hz-5079R LASS4  长寿相关基因lASS4抗体

hz-5082R LASS5  长寿相关基因lASS5抗体

hz-5080R LASS6  长寿相关基因lASS6抗体

hz-1972R LCAT  卵磷酯胆固醇酰基转移酶抗体

hz-2904R LATS1  肿瘤抑制基因LATS1抗体

hz-3245R Phospho-LATS1 (Thr1079)  磷酸化肿瘤抑制基因LATS1抗体

hz-3246R Phospho-LATS1 (Ser909)  磷酸化肿瘤抑制基因LATS1抗体

hz-4081R LATS2  肿瘤抑制基因LATS2抗体

hz-4082R phospho-LATS2 (Ser83)  磷酸化肿瘤抑制基因LATS2抗体

hz-0619R L-Citrulline  L-瓜氨酸抗体

hz-2649R Lck/p56-LCK  淋巴细胞特异性蛋白酪氨酸激酶抗体

hz-3255R Phospho-Lck (Tyr505)  磷酸化淋巴细胞特异性蛋白酪氨酸激酶抗体

hz-2200R LDL  低密度脂蛋白抗体

hz-0705R LDL receptor  低密度脂蛋白受体抗体

hz-1698R ox-LDL  氧化低密度脂蛋白抗体

hz-1810R LDHA/LDH  乳酸脱氢酶抗体

hz-3827R LDHC/Cancer,testis antigen 32  乳酸脱氢酶LDH-C（肿瘤/睾丸抗原32）抗体

hz-1582R Mannan Binding Lectin  甘露聚糖结合凝集素抗体

hz-1843R LEF-1  淋巴增强因子-1抗体

hz-3907R Legumain  半胱氨酸蛋白酶抗体

hz-0108R Leptin  瘦素抗体

hz-0409R Leptin  瘦素抗体

hz-4604R Leptin  瘦素抗体

hz-0410R Leptin receptor/Ob-R  瘦素受体抗体

hz-0109R Leptin receptor(long)  瘦素受体抗体（长）

hz-0961R Leptin receptor/OB-R  瘦素受体抗体

hz-1758R L-enk  亮氨酸脑啡肽抗体

hz-1117R GPR49/LGR5  G蛋白偶联受体49抗体

hz-4206R sLOX 1  可溶性凝集素样氧化低密度脂蛋白受体1抗体

hz-5789R GPR39  G蛋白偶联受体39抗体

hzt-0506M LH  人促黄体生成素抗体