获取电话英文名称 AP180
中文名称 装配衔接蛋白180抗体
别 名 AP 180; AP3; Assembly Protein 180 kDa; Assembly Protein AP180; CALM; Clathrin coat assembly protein; Clathrin coat assembly protein AP180; Clathrin coat associated protein AP180; DKFZp781O051; F120; KIAA0656; NP185; pp155; SNAP 91; SNAP91; 91kDa synaptosomal associated protein; Synaptosomal associated protein 91 kD homolog; Synaptosomal associated protein 91 kDa homolog; AP180_HUMAN.
装配衔接蛋白180抗体
说 明 书 0.2ml
研究领域 细胞生物 神经生物学 细胞类型标志物
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 92kDa
细胞定位 细胞浆 细胞膜
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human AP180
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 装配衔接蛋白180抗体 产品介绍 background:
Clathrin-coated pits and vesicles are assembled for receptor-mediated endocytosis through interaction with Clathrin associated protein complexes. Vesicle transport is mediated from the trans-Golgi network by the adapter complex AP-1 and from the plasma membrane by the AP-2 complex. The AP-1 and AP-2 adapter protein complexes consist of Clathrin binding Adaptin proteins (g and b1 for AP-1, a and b2 for AP-2) and two smaller subunits known as AP50 and AP17. The a and b Adaptin chains have a similar two-domain organization with C-terminal domains that vary in both sequence and length. a-Adaptin splice variants A and C display variable relative expression levels and differential distribution in different tissues. AP-3 (also designated AP180 or F1-20) is a synapse-specific Clathrin assembly protein. The protein CALM (Clathrin assembly protein lymphoid myeloid leukemia) is highly homologous to AP180 and may also be involved in Clathrin assembly.
Function:
AP180 is a clathrin binding phospho-protein and faciliates the formation of clathrin coats. This protein is also known as pp155, NP185, F1-20, and AP-3. During neurotransmitter release synaptic vesicles fuse with the presynaptic plasma membrane. A whole protein machinery consisting of amphiphysin, clathrin, endophilin and synaptojanin is involved in the subsequent endocytotic recycling of the synaptic vesicles.
Subunit:
Binds AP2A2. Interacts with AP2B1; clathrin competes with SNAP91 (By similarity).
Subcellular Location:
Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane.
Post-translational modifications:
Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity).
Similarity:
Contains 1 ENTH (epsin N-terminal homology) domain.
Database links:
Entrez Gene: 9892 Human
Entrez Gene: 20616 Mouse
Entrez Gene: 65178 Rat
Omim: 607923 Human
SwissProt: O60641 Human
SwissProt: Q61548 Mouse
SwissProt: Q05140 Rat
Unigene: 368046 Human
Unigene: 281651 Mouse
Unigene: 11022 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
装配衔接蛋白180抗体
hz-5484R phospho-ERK5(Ser496) 磷酸化细胞外信号调节激酶5抗体
hz-5486R phospho-ERK5( Ser731+Thr733) 磷酸化细胞外信号调节激酶5抗体
hz-1851R DUSP1/MKP1 丝裂原活化蛋白激酶磷酸酶-1抗体
hz-3278R Phospho-MKP1 (Ser359) 磷酸化丝裂原活化蛋白激酶磷酸酶-1抗体
hz-3279R Phospho-MKP1 (Ser296 + Ser318) 磷酸化丝裂原活化蛋白激酶磷酸酶-1抗体
hz-1852R MKP-2/DUSP4 丝裂原活化蛋白激酶磷酸酶-2抗体
hz-0122R ER-α 雌激素受体α抗体
hzm-1591M ER/PR/c-erbB-2 Kit ER/PR/c-erbB-2检测试剂盒（鼠单抗）
hz-0894R Phospho-Estrogen Receptor alpha (Ser167) 磷酸化雌激素受体ER alpha 抗体
hz-1643R Phospho-Estrogen Receptor alpha (Tyr537) 磷酸化雌激素受体α抗体
hz-3130R Phospho-Estrogen Receptor alpha (Ser118) 磷酸化雌激素受体α抗体
hz-3131R Phospho-Estrogen Receptor alpha (Ser104/106) 磷酸化雌激素受体α抗体
hz-5338R Phospho-Estrogen Receptor alpha (Ser305) 磷酸化雌激素受体α抗体
hz-0254R ER-Alpha 雌激素受体α抗体（用于western blot）
hz-5895R WASF1/WAVE 1 WAVE1蛋白抗体
hz-6243R WASF2 WASF2抗体
hz-2098R ER-Alpha 雌激素受体α抗体
hz-0174R ER-alpha/beta 雌激素受体α/β抗体
hz-5896R WFDC5 P53应答基因蛋白5抗体
hz-5899R HIFPH4 缺氧诱导因子脯氨酰4羟化酶抗体
hz-5900R ADORA2B 腺苷A2b受体抗体（神经生长因子1受体）
hz-2927R OXSR1 氧化应激反应蛋白1抗体
hz-0116R ER-beta 雌激素受体β 抗体
hz-0255R ER β 雌激素受体β抗体
hz-5901R OS9 OS-9蛋白抗体
hz-5913R Calreticulin 钙网蛋白抗体
hz-5902R RBM3 RNA结合基因蛋白3抗体
hz-0593R EpCAM/CD326 上皮特异性抗原抗体
hz-3615R ESM1 内皮细胞特异性分子1抗体
hz-4101R ESPL1 外纺锤体极样蛋白1抗体
hz-5304R phospho-ESPL1 (Ser1073) 磷酸化外纺锤体极样蛋白1抗体
hz-5121R ERp29 内质网蛋白29抗体