大鼠晚期糖基化终末产物受体(AGER)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠晚期糖基化终末产物受体(AGER)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠晚期糖基化终末产物受体(AGER)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠晚期糖基化终末产物受体(AGER)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠晚期糖基化终末产物受体(AGER)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB0746c 兔胃泌素释放肽前体(ProGRP)检测试剂盒 Rabbit ProGRP (Pro-Gastrin Releasing Peptide) ELISA Kit
HZ-EL-RB0747c 兔血小板相关抗体IgM(PA-IgM)检测试剂盒 Rabbit PA-IgM (Platelet Associated Antibody) ELISA Kit
HZ-EL-RB0748c 兔囊性纤维化跨膜传导调节因子(CFTR)检测试剂盒 Rabbit CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit
HZ-EL-RB0750c 兔抗胰岛素受体抗体(AIRA)检测试剂盒 Rabbit AIRA (Anti-Insulin Receptor Antibody) ELISA Kit
HZ-EL-RB0751c 兔蛋白C抑制剂(PCI)检测试剂盒 Rabbit PCI (Protein C Inhibitor ) ELISA Kit
HZ-EL-RB0752c 兔葡萄糖激酶调节蛋白(GKRP)检测试剂盒 Rabbit GKRP (Glucokinase Regulatory Protein) ELISA Kit
HZ-EL-RB0753c 兔失调症蛋白质1(ATXN1)检测试剂盒 Rabbit ATXN1 (Ataxin 1) ELISA Kit
HZ-EL-RB0754c 兔破骨细胞相关受体(OSCAR)检测试剂盒 Rabbit OSCAR (Osteoclast Associated Receptor) ELISA Kit
HZ-EL-RB0755c 兔补体成分7(C7)检测试剂盒 Rabbit C7 (Complement Component 7) ELISA Kit
HZ-EL-RB0756c 兔ras同源物基因家族成员a(RHOA)检测试剂盒 Rabbit RHOA (Ras Homolog Gene Family, Memmber A) ELISA Kit
HZ-EL-RB0757c 兔活化蛋白C(APC)检测试剂盒 Rabbit APC (Activated Protein C) ELISA Kit
HZ-EL-RB0758c 兔间羟肾上腺素(MN)检测试剂盒 Rabbit MN (Metanephrine) ELISA Kit
HZ-EL-RB0759c 兔胱天蛋白酶9(CASP9)检测试剂盒 Rabbit CASP 9 (Caspase 9) ELISA Kit
HZ-EL-RB0762c 兔AMP活化蛋白激酶α1(PRKAα1)检测试剂盒 Rabbit PRKAα1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit
HZ-EL-RB0765c 兔间隙连接蛋白(Cx)检测试剂盒 Rabbit Cx (Connexin) ELISA Kit
HZ-EL-RB0767c 兔非受体酪氨酸激酶2(TNK2)检测试剂盒 Rabbit TNK2 (Tyrosine Kinase, Non Receptor 2) ELISA Kit
HZ-EL-RB0768c 兔蛋白酶3抗体(PR3Ab)检测试剂盒 Rabbit PR3Ab (Proteinase 3 Antibody) ELISA Kit
HZ-EL-RB0769c 兔白介素11(IL-11)检测试剂盒 Rabbit IL-11 (Interleukin 11) ELISA Kit
HZ-EL-RB0772c 兔胃蛋白酶原A(PGA)检测试剂盒 Rabbit PGA (Pepsinogen A ) ELISA Kit
HZ-EL-RB0773c 兔锌结合α2-糖蛋白1(αZGP1)检测试剂盒 Rabbit αZGP1 (Alpha-2-Glycoprotein 1, Zine Binding) ELISA Kit
HZ-EL-RB0774c 兔封闭蛋白9(CLDN9)检测试剂盒 Rabbit CLDN9 (Claudin 9) ELISA Kit
HZ-EL-RB0775c 兔γ谷氨酰转移酶1(γGT1)检测试剂盒 Rabbit γGT1 (Gamma Glutamyltransferase 1) ELISA Kit
HZ-EL-RB0776c 兔血管紧张素II受体2抗体(ANGⅡR2-Ab)检测试剂盒 Rabbit ANG II R2-Ab (Angiotensin II Receptor 2 Antibody) ELISA Kit
HZ-EL-RB0777c 兔核因子κB抑制蛋白α(IκBα)检测试剂盒 Rabbit IκBα (Inhibitory Subunit of NF Kappa B Alpha) ELISA Kit
HZ-EL-RB0778c 兔纤溶酶-抗纤溶酶复合物(PAP)检测试剂盒 Rabbit PAP (Plasmin-Antiplasmin Complex) ELISA Kit
HZ-EL-RB0779c 兔高分子量细胞角蛋白(CK-HMW)检测试剂盒 Rabbit CK-HMW (Cytokeratin High Molecular Weight) ELISA Kit
HZ-EL-RB0780c 兔3-羟基-3-甲基戊二酰辅酶A合酶1(HMGCS1)检测试剂盒 Rabbit HMGCS1 (3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1) ELISA Kit
HZ-EL-RB0781c 兔嗜酸粒细胞趋化因子(ECF/CCL11)检测试剂盒 Rabbit ECF/CCL11 (Eosinophil Chemotactic Factor) ELISA Kit