大鼠糖链抗原15-3(CA15-3)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠糖链抗原15-3(CA15-3)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠糖链抗原15-3(CA15-3)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠糖链抗原15-3(CA15-3)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠糖链抗原15-3(CA15-3)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-RB0332c 兔β-促脂素(β-LPH)检测试剂盒 Rabbit β-LPH (β-Lipotropic Hormone) ELISA Kit
HZ-EL-RB0333c 兔胞浆型磷脂酶A2(cPLA2)检测试剂盒 Rabbit cPLA2 (Cytosolic Phospholipase A2) ELISA Kit
HZ-EL-RB0334c 兔钙网蛋白(CRT)检测试剂盒 Rabbit CRT (Calreticulin) ELISA Kit
HZ-EL-RB0336c 兔中脑星型胶质细胞来源神经营养因子(MANF)检测试剂盒 Rabbit MANF (Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit
HZ-EL-RB0337c 兔白介素19(IL-19)检测试剂盒 Rabbit IL-19 (Interleukin 19) ELISA Kit
HZ-EL-RB0339c 兔血清淀粉样蛋白A2(SAA2)检测试剂盒 Rabbit SAA2 (Serum Amyloid A2) ELISA Kit
HZ-EL-RB0340c 兔内皮型一氧化氮合酶(NOS3/eNOS)检测试剂盒 Rabbit NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) ELISA Kit
HZ-EL-RB0341c 兔血浆抗凝蛋白C(PC)检测试剂盒 Rabbit PC (Plasma Anticoagulant Protein C) ELISA Kit
HZ-EL-RB0342c 兔音猬因子(SHH )检测试剂盒 Rabbit SHH (Hedgehog Homolog, Sonic) ELISA Kit
HZ-EL-RB0343c 兔丝氨酸蛋白酶1(PRSS1)检测试剂盒 Rabbit PRSS1 (Protease, Serine, 1) ELISA Kit
HZ-EL-RB0344c 兔癌蛋白诱导转录物3(OIT3)检测试剂盒 Rabbit OIT3 (Oncoprotein Induced Transcript 3) ELISA Kit
HZ-EL-RB0345c 兔接触蛋白相关蛋白样蛋白2(CNTNAP2)检测试剂盒 Rabbit CNTNAP2 (Contactin Associated Protein Like Protein 2) ELISA Kit
HZ-EL-RB0346c 兔重肽铁蛋白(FTH)检测试剂盒 Rabbit FTH (Ferritin, Heavy Polypeptide) ELISA Kit
HZ-EL-RB0348c 兔低密度脂蛋白受体相关蛋白6(LRP6)检测试剂盒 Rabbit LRP6 (Low Density Lipoprotein Receptor Related Protein) ELISA Kit
HZ-EL-RB0349c 兔基质金属蛋白酶1(MMP1)检测试剂盒 Rabbit MMP-1 (Matrix Metalloproteinase 1) ELISA Kit
HZ-EL-RB0350c 兔胎盘蛋白(PP)检测试剂盒 Rabbit PP (Placental Protein) ELISA Kit
HZ-EL-RB0351c 兔性别决定区Y框蛋白1(SOX1)检测试剂盒 Rabbit SOX1 (Sex Determining Region Y Box Protein 1) ELISA Kit
HZ-EL-RB0352c 兔卵清蛋白特异性IgG(OVA sIgG)检测试剂盒 Rabbit OVA sIgG (Ovalbumin Specific IgG) ELISA Kit
HZ-EL-RB0353c 兔白三烯D4(LTD4)检测试剂盒 Rabbit LTD4 (Leukotriene D4) ELISA Kit
HZ-EL-RB0355c 兔反三碘甲状腺原氨酸(rT3)检测试剂盒 Rabbit rT3 (Reverse Triiodothyronine) ELISA Kit
HZ-EL-RB0356c 兔心肌营养素合成酶/心磷脂合酶(CLS)检测试剂盒 Rabbit CLS (Cardiolipin Synthase) ELISA Kit
HZ-EL-RB0357c 兔蛋白Z(PROZ)检测试剂盒 Rabbit PROZ (Protein Z) ELISA Kit
HZ-EL-RB0358c 兔胎盘蛋白13 (PP13)检测试剂盒 Rabbit PP13 (Placental Protein13) ELISA Kit