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一键询价大鼠纤维连接蛋白(FN)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠纤维连接蛋白(FN)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠纤维连接蛋白(FN)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠纤维连接蛋白(FN)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠纤维连接蛋白(FN)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK1634c 猴α谷胱甘肽S转移酶(α-GST)检测试剂盒 Monkey α-GST (α-glutathione S-transferases) ELISA Kit
HZ-EL-MK1635c 猴凝血因子Ⅹ(F10)检测试剂盒 Monkey F10 (Coagulation Factor X) ELISA Kit
HZ-EL-MK1636c 猴转化生长因子α(TGF-α)检测试剂盒 Monkey TGF-α (Transforming Growth Factor α) ELISA Kit
HZ-EL-MK1637c 猴胞外超氧化物歧化酶(SOD3)检测试剂盒 Monkey SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit
HZ-EL-MK1638c 猴囊性纤维化跨膜传导调节因子(CFTR)检测试剂盒 Monkey CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit
HZ-EL-MK1639c 猴促胃液素受体(GasR)检测试剂盒 Monkey GasR (Gastrin Receptor) ELISA Kit
HZ-EL-MK1640c 猴血小板膜糖蛋白VI(GP6)检测试剂盒 Monkey GP6 (Glycoprotein VI, Platelet) ELISA Kit
HZ-EL-MK1641c 猴黄体生成素释放激素(LHRH/GnRH)检测试剂盒 Monkey LHRH/GnRH (Luteinizing Hormone Releasing Hormone) ELISA Kit
HZ-EL-MK1642c 猴胰蛋白酶(TRY)检测试剂盒 Monkey TRY (Trypsin) ELISA Kit
HZ-EL-MK1644c 猴乳酸脱氢酶B(LDHB)检测试剂盒 Monkey LDHB (Lactate Dehydrogenase B) ELISA Kit
HZ-EL-MK1645c 猴端锚聚合酶1 (TNKS1)检测试剂盒 Monkey TNKS1 (Tankyrase 1) ELISA Kit
HZ-EL-MK1646c 猴甘露糖受体C1(MRC1)检测试剂盒 Monkey MRC1 (Mannose Receptor C Type 1 ) ELISA Kit
HZ-EL-MK1647c 猴生长停滞特异性蛋白6(Gas6)检测试剂盒 Monkey Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
HZ-EL-MK1648c 猴核因子κB (NFκB)检测试剂盒 Monkey NFκB (Nuclear Factor Kappa B) ELISA Kit
HZ-EL-MK1649c 猴八聚体转录因子2B(OTF2B)检测试剂盒 Monkey OTF2B (Octamer Transcription Factor 2B) ELISA Kit
HZ-EL-MK1650c 猴腺苷酸环化酶相关蛋白1(CAP1)检测试剂盒 Monkey CAP1 (Adenylate Cyclase Associated Protein 1) ELISA Kit
HZ-EL-MK1651c 猴细胞因子信号转导抑制因子3(SOCS3)检测试剂盒 Monkey SOCS3 (Suppressors of Cytokine Signaling 3) ELISA Kit
HZ-EL-MK1652c 猴细胞粘附蛋白相互作用蛋白(CYTIP)检测试剂盒 Monkey CYTIP (Cytohesin Interacting Protein) ELISA Kit
HZ-EL-MK1653c 猴T细胞生长因子-Ⅲ(TCGF-Ⅲ)检测试剂盒 Monkey TCGF-Ⅲ (Tcell Growth Factor Ⅲ) ELISA Kit
HZ-EL-MK1654c 猴成纤维细胞生长因子结合蛋白2(FGFBP2)检测试剂盒 Monkey FGFBP2 (Fibroblast Growth Factor Binding Protein 2) ELISA Kit
HZ-EL-MK1655c 猴酸性磷酸酶1(ACP1)检测试剂盒 Monkey ACP1 (Acid Phosphatase 1) ELISA Kit
HZ-EL-MK1656c 猴碳酸酐酶IX (CA9)检测试剂盒 Monkey CA9 (Carbonic Anhydrase IX) ELISA Kit
HZ-EL-MK1657c 猴脑型肌酸激酶(CKB)检测试剂盒 Monkey CKB (Creatine Kinase, Brain) ELISA Kit
HZ-EL-MK1658c 猴肠脂肪酸结合蛋白(IFABP)检测试剂盒 Monkey IFABP/FABP2 (Intestinal Fatty Acid Binding Protein) ELISA Kit
HZ-EL-MK1659c 猴泛素结合酶E2C(UBE2C)检测试剂盒 Monkey UBE2C (Ubiquitin Conjugating Enzyme E2C) ELISA Kit
HZ-EL-MK1660c 猴趋化因子CXC配体16 (CXCL16)检测试剂盒 Monkey CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit