早期有丝分裂抑制蛋白1抗体

英文名称    Emi1    

中文名称    早期有丝分裂抑制蛋白1抗体    

别    名    Early mitotic inhibitor 1; Emi 1; F box only protein 5; F box protein 5; F box protein Fbx 5; F box protein Fbx5; F-box only protein 5; FBX5; FBX5_HUMAN; fbxo5.    

说 明 书    0.2ml      

研究领域    信号转导  泛素      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 早期有丝分裂抑制蛋白1抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    50kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Emi1    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

早期有丝分裂抑制蛋白1抗体产品介绍    background:

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is similar to xenopus early mitotic inhibitor-1 (Emi1), which is a mitotic regulator that interacts with Cdc20 and inhibits the anaphase promoting complex. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Dec 2008]

Function:
Regulates progression through early mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. Can also bind directly to the APC to inhibit substrate-binding.

Subcellular Location:
Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. In interphase, localizes in a punctate manner in the nucleus and cytoplasm with some perinuclear concentration. In mitotic cells, localizes throughout the cell, particularly at the spindle.

Post-translational modifications:
Phosphorylation by CDK2 and subsequently by PLK1 triggers degradation during early mitosis through ubiquitin-mediated proteolysis by the SCF ubiquitin ligase complex containing the F-box protein BTRC. This degradation is necessary for the activation of APC in late mitosis and subsequent mitotic progression.

Similarity:
Contains 1 F-box domain.
Contains 1 IBR-type zinc finger.

Gene ID:
26271

Database links:

Entrez Gene: 26271 Human

GenBank: BC018905 Human

Omim: 606013 Human

SwissProt: Q9UKT4 Human

Unigene: 520506 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

早期有丝分裂抑制蛋白1抗体

hz-3245R Phospho-LATS1 (Thr1079)  磷酸化肿瘤抑制基因LATS1抗体

hz-3246R Phospho-LATS1 (Ser909)  磷酸化肿瘤抑制基因LATS1抗体

hz-4081R LATS2  肿瘤抑制基因LATS2抗体

hz-4082R phospho-LATS2 (Ser83)  磷酸化肿瘤抑制基因LATS2抗体

hz-0619R L-Citrulline  L-瓜氨酸抗体

hz-2649R Lck/p56-LCK  淋巴细胞特异性蛋白酪氨酸激酶抗体

hz-3255R Phospho-Lck (Tyr505)  磷酸化淋巴细胞特异性蛋白酪氨酸激酶抗体

hz-2200R LDL  低密度脂蛋白抗体

hz-0705R LDL receptor  低密度脂蛋白受体抗体

hz-1698R ox-LDL  氧化低密度脂蛋白抗体

hz-1810R LDHA/LDH  乳酸脱氢酶抗体

hz-3827R LDHC/Cancer,testis antigen 32  乳酸脱氢酶LDH-C（肿瘤/睾丸抗原32）抗体

hz-1582R Mannan Binding Lectin  甘露聚糖结合凝集素抗体

hz-1843R LEF-1  淋巴增强因子-1抗体

hz-3907R Legumain  半胱氨酸蛋白酶抗体

hz-0108R Leptin  瘦素抗体

hz-0409R Leptin  瘦素抗体

hz-4604R Leptin  瘦素抗体

hz-0410R Leptin receptor/Ob-R  瘦素受体抗体

hz-0109R Leptin receptor(long)  瘦素受体抗体（长）

hz-0961R Leptin receptor/OB-R  瘦素受体抗体

hz-1758R L-enk  亮氨酸脑啡肽抗体

hz-1117R GPR49/LGR5  G蛋白偶联受体49抗体

hz-4206R sLOX 1  可溶性凝集素样氧化低密度脂蛋白受体1抗体

hz-5789R GPR39  G蛋白偶联受体39抗体

hzt-0506M LH  人促黄体生成素抗体

hz-0951R LH beta  促黄体生成素抗体

hz-0952R LH  促黄体生成素抗体

hz-0984R LHR/CGR  促黄体生成素受体抗体

hz-1054R HDC  L-组氨酸脱羧酶抗体

hz-0456R LHRH/GNRH  黄体激素释放激素类似物抗体

hz-1058R LIF  白血病抑制因子抗体

hz-1458R LIFR/CD118  白血病抑制因子受体抗体