大鼠糖化血红蛋白(HbA1c)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠糖化血红蛋白(HbA1c)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠糖化血红蛋白(HbA1c)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠糖化血红蛋白(HbA1c)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠糖化血红蛋白(HbA1c)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-P0044c 猪甲酰肽受体2(FPR2)检测试剂盒 Porcine FPR2 (Formyl Peptide Receptor 2) ELISA Kit
HZ-EL-P0048c 猪脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒 Porcine LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
HZ-EL-P0049c 猪睫状神经营养因子(CNTF)检测试剂盒 Porcine CNTF (Ciliary Neurotrophic Factor) ELISA Kit
HZ-EL-P0050c 猪血小板衍生生长因子AA(PDGF-AA)检测试剂盒 Porcine PDGF-AA (Platelet Derived Growth Factor-AA) ELISA Kit
HZ-EL-P0051c 猪CCAAT增强子结合蛋白β(C/EBPβ)检测试剂盒 Porcine C/EBPβ (CCAAT/Enhancer Binding Protein Beta) ELISA Kit
HZ-EL-P0052c 猪转录激活因子-2(ATF-2)检测试剂盒 Porcine ATF-2 (Activating Transcription Factor 2) ELISA Kit
HZ-EL-P0053c 猪粘蛋白17(MUC17)检测试剂盒 Porcine MUC17 (Mucin 17) ELISA Kit
HZ-EL-P0054c 猪细胞外信号调节激酶3(ERK3)检测试剂盒 Porcine ERK3 (Extracellular Signal Regulated Kinase 3) ELISA Kit
HZ-EL-P0055c 猪尿激酶型纤溶酶原激活因子(PLAU/uPA)检测试剂盒 Porcien PLAU/uPA(Urokinase-Type Plasminogen Activator) ELISA Kit
HZ-EL-P0056c 猪血管性血友病因子(VWF)检测试剂盒 Porcine VWF (Von Willebrand Factor) ELISA Kit
HZ-EL-P0057c 猪绒毛膜促性腺激素α肽(CGα)检测试剂盒 Porcine CGα (Chorionic Gonadotropin Alpha Polypeptide) ELISA Kit
HZ-EL-P0058c 猪神经胶质纤维酸性蛋白(GFAP)检测试剂盒 Porcine GFAP (Glial Fibrillary Acidic Protein) ELISA Kit
HZ-EL-P0059c 猪成纤维细胞生长因子7(FGF7/KGF)检测试剂盒 Porcine FGF-7/KGF (Fibroblast Growth Factor 7) ELISA Kit
HZ-EL-P0060c 猪粘蛋白13(MUC13)检测试剂盒 Porcine MUC13 (Mucin 13) ELISA Kit
HZ-EL-P0061c 猪氨基端前脑钠素(NT-proBNP)检测试剂盒 Porcine NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
HZ-EL-P0063c 猪瘦素(LEP)检测试剂盒 Porcine LEP (Leptin) ELISA Kit
HZ-EL-P0065c 猪血小板相关补体3(PAC3)检测试剂盒 Porcine PAC3 (Platelet Associated Complement 3) ELISA Kit
HZ-EL-P0066c 猪前列腺特异性抗原(PSA/KLK3)检测试剂盒 Porcine PSA/KLK3 (Prostate Specific Antigen) ELISA Kit
HZ-EL-P0068c 猪半乳凝集素1(GAL1)检测试剂盒 Porcine GAL1 (Galectin 1 ) ELISA Kit
HZ-EL-P0069c 猪VI型胶原(COL6)检测试剂盒 Porcine COL6 (Collagen Type VI) ELISA Kit
HZ-EL-P0070c 猪细胞毒性T淋巴细胞相关抗原4(CTLA4)检测试剂盒 Porcine CTLA4 (Cytotoxic T Lymphocyte Associated Antigen 4) ELISA Kit
HZ-EL-P0071c 猪苯丙酸诺龙/苯丙酸去甲睾酮(NP)检测试剂盒 Porcine NP (Nandrolone Phenylpropionate) ELISA Kit
HZ-EL-P0072c 猪B细胞淋巴瘤因子2(Bcl-2)检测试剂盒 Porcine Bcl-2 (B-cell Leukemia/Lymphoma 2) ELISA Kit
HZ-EL-P0073c 猪凝血因子ⅩⅢ(F13)检测试剂盒 Porcine F13 (Coagulation Factor ⅩⅢ) ELISA Kit
HZ-EL-P0074c 猪结缔组织活化肽Ⅲ(CTAPⅢ)检测试剂盒 Porcine CTAPIII (Connective Tissue Activating Peptide III) ELISA Kit
HZ-EL-P0075c 猪促生长激素(GH)检测试剂盒 Porcine GH (Growth Hormone) ELISA Kit
HZ-EL-P0076c 猪胎盘蛋白13 (PP13)检测试剂盒 Porcine PP13 (Placental Protein13) ELISA Kit
HZ-EL-P0077c 猪载脂蛋白B100(ApoB100)检测试剂盒 Porcine ApoB100 (Apolipoprotein B100) ELISA Kit