眼睛发育缺失蛋白EYA2抗体

英文名称    EYA2    

中文名称    眼睛发育缺失蛋白EYA2抗体    

别    名    EAB 1; EAB1; EYA 2; EYA2; EYA2_HUMAN; eyes absent 2 homolog (Drosophila); Eyes absent homolog 2; Translation of this uORF probably lowers the translation efficiency of EYA2.    

说 明 书    0.1ml  0.2ml      

研究领域    细胞生物  发育生物学  神经生物学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 眼睛发育缺失蛋白EYA2抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    59kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EYA2    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

眼睛发育缺失蛋白EYA2抗体产品介绍    background:

A gene on chromosome 20q13.1 encodes Eya2 (eyes absent). EYA2 is one of four members of the eyes absent family. A 271 amino acid domain at the carboxy-terminal is highly conserved amongst the members of the eyes absent family, while the PST (proline-serive-threonin)-rich amino-terminal is highly divergent. EYA2 is expressed relatively late in development in the cytoplasm of extensor tendons and ligaments of the phalangeal elements of the limb, cranial placodes, branchial arches, central nervous system, and the developing eye. Pax3 induces the expression of Eya2 in a cascade that is necessary and sufficient for myogenesis. EYA2, like EYA1, acts as a transcriptional activator in connective tissue patterning through its PST domain, which functions as a transactivation domain. EYA2 is translocated to the nucleus by Six proteins, which interact through their domain and homeodomain with EYA2. EYA2 carboxy-terminal interacts with the G Alpha z and G Alphai 2 proteins. This interaction prevents Six proteins from translocating EYA2 to the nucleus.

Function:
Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Coactivates SIX1. Seems to coactivate SIX2, SIX4 and SIX5. Together with SIX1 and DACH2 seem to be involved in myogenesis. May be involved in development of the eye. Interaction with GNAZ and GNAI2 prevents nuclear translocation and transcriptional activity. 

Subunit:
Interacts with GNAZ and GNAI2. Interacts with DACH2 and SIX1, and probably with SIX2, SIX4 and SIX5. Interacts with CAPN8 (By similarity). 

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Highest expression in muscle with lower levels in kidney, placenta, pancreas, brain and heart.

Similarity:
Belongs to the HAD-like hydrolase superfamily. EYA family.

Database links:
UniProtKB/Swiss-Prot: O00167.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.    

眼睛发育缺失蛋白EYA2抗体

hz-1839P lamin A  核纤层蛋白A抗原

hz-2044P LOX-1/OLR1  凝集素样氧化型低密度脂蛋白受体

hz-0976P LPLUNC1  人鼻咽癌癌基因多肽抗原

hz-1311P LYVE-1(lymphalic vessel endotheilial hyaluronan receptor 1)  淋巴管内皮透明质酸受体抗原

hz-0439P ACE( ACE, testis-specific isoform precursor)  血管紧张素转换酶(抗原)

hz-2217P Integrin alpha E2 peptide  整合素alpha E2抗原

hz-1014P Mac-1 (Mac-1/CR3/CD11b+CD18)  巨噬细胞表面分子抗原

hz-0924P Mafa(avian)(V-maf musculoaponeurotic fibrosarcoma oncogene homolog A)  v-maf 肌腱膜纤维肉瘤癌基因同源物A抗原

hz-0817P MAGE-1  黑色素瘤相关

hz-1041P MAPKK1 (MAP kinase kinase 1)  丝裂原活化蛋白激酶激酶1抗原

hz-1425P ASK1/MAPKKK5  细胞凋亡信号调节激酶1/丝裂原活化蛋白激酶激酶激酶5抗原

hz-0792P Maspin (mammary serine protease inhibitor)  抑癌基因抗原

hz-0531P Matriptase  蛋白裂解酶（一种新的癌基因）抗原

hz-0808P HCMV UL49(Human Cytomegalovirus UL49 Gene)  人巨细胞病毒UL49抗原

hz-1419P MC-1R (melanocortin-1-receptor)  黑皮质素-1受体抗原

hz-1352P Mcl-1(myeloid cell leukhzia 1) peptide  髓样细胞白血病-1抗原

hz-1043P MDM2 (urine double minute 2)  双微体2癌基因抗原

hz-1468P MDR1(multidrug resistance 1)  多药耐药蛋白抗原

hz-1400P MEF2(myocyte enhancer-binding factor 2)  肌细胞增强因子2抗原

hz-0557P Mfn1 (Mitofusin1)  线粒体融合蛋白1

hz-1328P MT(metallothionein)  金属硫蛋白抗原

hz-0900P MGr1-Ag/37LRP(P37-kDa laminin receptor precursor)  层粘连蛋白受体1抗体

hz-0901P MGr1-Ag/37LRP(P37-kDa laminin receptor precursor)(CT)  层粘连蛋白受体1抗原（C端）

hz-1849P MK(Midkine)  中期因子/肝素结合生长因子抗原

hz-2240P MLC/Myosin light chain 3 peptide  肌球蛋白轻链3抗原

hz-1044P MIF (Macrophage Migration Inhibitory Factor)  巨噬细胞移动抑制因子抗原

hz-1046P MIP-1 Beta (macrophage inflammatory protein 1 Beta)  巨噬细胞炎症因子1β 抗原

hz-1959P CD122/IL-2RB(Imterleukm-2 Receptor beta)  CD122/白介素2受体β链抗原

hz-1162P MIP2 (myocardial ischhzic preconditioning upregulated protein 2)  巨噬细胞炎症蛋白2抗原

hz-0575P MMP-13 (Matrix metalloproteinase 13)  基质金属蛋白酶13抗原

hz-0985P MMP-20(matrix metalloproteinase 20)  基质金属蛋白酶20抗原

hz-1862P MMP-17(Matrix metalloproteinase-17)  基质金属蛋白酶-17抗原