鸟嘌呤核苷酸交换因子4

英文名称    Epac2    

中文名称    鸟嘌呤核苷酸交换因子4    

别    名    CAMP GEFII; cAMP regulated guanine nucleotide exchange factor II; cAMP-GEFII; cAMP-regulated guanine nucleotide exchange factor II; CAMPGEFII; CGEF 2; CGEF2; Epac 2; EPAC2; Exchange factor directly activated by cAMP 2; Exchange protein directly activated by cAMP 2; Nbla00496; Putative protein product of Nbla00496; Rap guanine nucleotide exchange factor (GEF) 4; RAP guanine nucleotide exchange factor 4; RAPGEF 4; RAPGEF4; RPGF4_HUMAN.    

说 明 书    0.2ml      

研究领域    细胞生物  神经生物学  信号转导      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 鸟嘌呤核苷酸交换因子4（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    116kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human Epac2/RAPGEF4    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

鸟嘌呤核苷酸交换因子4产品介绍    background:

3',5' cyclic adenosine monophosphate (cAMP)-regulated guanine nucleotide exchange factors Epac1 (cAMP-GEFI) and Epac2 (cAMP-GEFII) activate the ras family GTPases Rap1 and Rap2 by promoting GTP binding in a cAMP-dependent manner (1,2). Eukaryotic cAMP is a second messenger that induces physiological responses such as gene expression, growth, differentiation, secretion and neurotransmission (3). Human EPAC2 contains at least 31 exons and maps to chromosome 2q31 (4). The 4.4-kb Epac2 transcript is prominent in brain and adrenal gland (5,6). Within the brain, expression is strong in cortex, occipital pole, frontal lobe, temporal lobe, amygdala, putamen, hippocampus and cerebellum (5,6). 

Function:
Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2.

Subunit:
Interacts with RIMS1 and RIMS2. Probably part of a complex with RIMS2 and GTP-activated RAB3A (By similarity). 

Subcellular Location:
Cytoplasm. Membrane.

Tissue Specificity:
Predominantly expressed in brain and adrenal gland. Isoform 2 is expressed in liver. Isoform 1 is expressed in liver at very low levels.

Similarity:
Contains 2 cyclic nucleotide-binding domains. Contains 1 DEP domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 Ras-GEF domain.    

鸟嘌呤核苷酸交换因子4

hz-0377R-AF647 Rabbit Anti-mouse IgG-Fc/Alexa Fluor 647  Alexa Fluor 647标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzy5.5 Rabbit Anti-mouse IgG-Fc/PE-Cy5.5  PE-Cy5.5标记的兔抗小鼠IgG-Fc

hz-0377R-PE-hzy7 Rabbit Anti-mouse IgG-Fc/PE-Cy7  PE-Cy7标记的兔抗小鼠IgG-Fc

hz-0377R-AP Rabbit Anti-mouse IgG-Fc/AP  碱性磷酸酶（AP）标记的兔抗小鼠IgG-Fc

hz-0377R-Gold Rabbit Anti-mouse IgG-Fc/Gold  胶体金标记的兔抗小鼠IgG-Fc

hz-0377R-hzy3 Rabbit Anti-mouse IgG-Fc/Cy3  Cy3标记的兔抗小鼠IgG-Fc

hz-0377R-hzy5 Rabbit Anti-mouse IgG-Fc/Cy5  Cy5标记的兔抗小鼠IgG-Fc

hz-0377R-hzy5.5 Rabbit Anti-mouse IgG-Fc/Cy5.5  Cy5.5标记的兔抗小鼠IgG-Fc

hz-0377R-hzy7 Rabbit Anti-mouse IgG-Fc/Cy7  Cy7标记的兔抗小鼠IgG-Fc

hz-0377R-PE Rabbit Anti-mouse IgG-Fc/PE  PE标记的兔抗小鼠IgG-Fc

hz-0377R-FIThz Rabbit Anti-mouse IgG-Fc/FITC  FITC标记的兔抗小鼠IgG-Fc

hz-0377R-RBIThz Rabbit Anti-mouse IgG-Fc/RBITC  罗丹明标记的兔抗小鼠IgG-Fc

hz-0377R-Bio Rabbit Anti-mouse IgG-Fc/Bio  生物素标记的兔抗小鼠IgG-Fc

hz-0335R-RBIThz Rabbit Anti-Monkey IgG/RBITC  罗丹明标记的兔抗猴IgG

hz-0335R-FIThz Rabbit Anti-Monkey IgG/FITC  FITC标记的兔抗猴IgG

hz-0335R-HRP Rabbit Anti-Monkey IgG/HRP  辣根过氧化物酶标记的兔抗猴IgG

hz-0335R-Gold Rabbit Anti-Monkey IgG/Gold  胶体金标记的兔抗猴IgG

hz-0335R-AP Rabbit Anti-Monkey IgG/AP  碱性磷酸酶（AP）标记的兔抗猴IgG

hz-0335R-Bio Rabbit Anti-Monkey IgG/Bio  生物素标记的兔抗猴IgG

hz-0335R-hzy3 Rabbit Anti-Monkey IgG/Cy3  Cy3标记的兔抗猴IgG

hz-0335R-hzy5 Rabbit Anti-Monkey IgG/Cy5  Cy5标记的兔抗猴IgG

hz-0335R-hzy5.5 Rabbit Anti-Monkey IgG/Cy5.5  Cy5.5标记的兔抗猴IgG

hz-0335R-hzy7 Rabbit Anti-Monkey IgG/Cy7  Cy7标记的兔抗猴IgG

hz-0335R-PE Rabbit Anti-Monkey IgG/PE  PE标记的兔抗猴IgG

hz-0335R-PE-hzy3 Rabbit Anti-Monkey IgG/PE-Cy3  PE-Cy3标记的兔抗猴IgG

hz-0335R-PE-hzY5 Rabbit Anti-Monkey IgG/PE-CY5  PE-CY5标记的兔抗猴IgG

hz-0335R-APhz Rabbit Anti-Monkey IgG/APC  APC标记的兔抗猴IgG

hz-0335R-AF350 Rabbit Anti-Monkey IgG/Alexa Fluor 350  Alexa Fluor 350标记的兔抗猴IgG

hz-0335R-AF488 Rabbit Anti-Monkey IgG/Alexa Fluor 488  Alexa Fluor 488标记的兔抗猴IgG

hz-0335R-AF555 Rabbit Anti-Monkey IgG/Alexa Fluor 555  Alexa Fluor 555标记的兔抗猴IgG

hz-0335R-AF647 Rabbit Anti-Monkey IgG/Alexa Fluor 647  Alexa Fluor 647标记的兔抗猴IgG

hz-0335R-PE-hzy5.5 Rabbit Anti-Monkey IgG/PE-Cy5.5  PE-Cy5.5标记的兔抗猴IgG