真核翻译起始因子2C2抗体

英文名称    eIF2C2    

中文名称    真核翻译起始因子2C2抗体    

别    名    Ago 2; AGO2_HUMAN; Argonaute 2; Argonaute2; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein.    

说 明 书    0.1ml  0.2ml      

研究领域    细胞生物  染色质和核信号  表观遗传学      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 真核翻译起始因子2C2抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    97kDa    

细胞定位    细胞核 细胞浆     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human eIF2C2/Ago2    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

真核翻译起始因子2C2抗体产品介绍    background:

Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Function:
Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.

Subunit:
Interacts with DICER1 through its Piwi domain and with TARBP2 during assembly of the RNA-induced silencing complex (RISC). Together, DICER1, EIF2C2/AGO2 and TARBP2 constitute the trimeric RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC). Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Note however that the term RISC has also been used to describe the trimeric RLC/miRLC. The formation of RISC complexes containing siRNAs rather than miRNAs appears to occur independently of DICER1. Interacts with EIF2C1/AGO1. Also interacts with DDB1, DDX5, DDX6, DDX20, DHX30, DHX36, DDX47, DHX9, EIF6, ELAVL, FXR1, GEMIN4, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, P4HA1, P4HB, RBM4, SART3, TNRC6A, TNRC6B, UPF1 and YBX1. Interacts with the P-body components DCP1A and XRN1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with RBM4; the interaction is modulated under stress-induced conditions, occurs under both cell proliferation and differentiation conditions and in a RNA-and phosphorylation-independent manner. Interacts with LIMD1, WTIP and AJUBA. Interacts with TRIM71.    

真核翻译起始因子2C2抗体

hz-1443R VCP  含缬酪肽蛋白抗体

hz-1461R VDAC  等电压依赖性阴离子通道抗体

hz-2806R VDCC-L alpha/CCHL1A1  L-型电压依赖型钙通道α抗体

hz-2996R CACNB2  L-型电压依赖型钙通道β抗体

hz-0279R VEGF  血管内皮生长因子抗体

hz-1313R VEGF  血管内皮生长因子抗体

hz-1957R VEGF-A/VEGF 165  血管内皮生长因子A抗体

hz-2638R PROK1/PRK1/EG-VEGF  内分泌腺衍生血管内皮生长因子抗体

hz-3347R Phospho-PRK1 (Thr774)/PRK2 (Thr816)  磷酸化内分泌腺衍生血管内皮生长因子抗体

hz-4203R PDZ RHOGEF/GTRAP48  Rho鸟甘酸转运蛋白抗体

hz-1586R VEGF-C  血管内皮生长因子C型抗体

hz-4572R VEGF-A  血管内皮生长因子A

hz-1915R VEGF-B  血管内皮生长因子B抗体

hz-2742R VEGF-D  血管内皮生长因子D型抗体

hz-0170R VEGFR1/FLT1  血管内皮生长因子受体1抗体

hz-0565R VEGFR2  血管内皮生长因子受体2抗体

hz-2674R phospho-VEGFR2(Tyr951)  磷酸化血管内皮生长因子受体2抗体

hz-3467R phospho-VEGF receptor 2 (Tyr1059)  磷酸化血管内皮生长因子受体2抗体

hz-3468R phospho-VEGFR2 (Tyr1175)  磷酸化血管内皮生长因子受体2抗体

hz-3469R phospho-VEGF receptor 2(Tyr1214)  磷酸化血管内皮生长因子受体2抗体

hz-3470R phospho-VEGF receptor 2 (Tyr996)  磷酸化血管内皮生长因子受体2抗体

hz-2089R sVEGFR2  可溶性血管内皮生长因子受体2抗体

hz-1083R VEGFR3  血管内皮细胞生长因子受体3抗体

hz-2202R VEGFR3  血管内皮细胞生长因子受体3抗体

hz-2533R Versican  蛋白聚糖Versican抗体

hz-3810R Villin  绒毛蛋白抗体

hz-0756R Vimentin  波形蛋白抗体

hz-3471R Phospho-Vimentin (Ser56)  磷酸化波形蛋白抗体

hz-3472R Phospho-Vimentin(Ser82)  磷酸化波形蛋白抗体

hz-0077R VIP  血管活性肠肽抗体

hz-3915R Vitamin D Binding protein  维生素D结合蛋白抗体

hz-0272R PBEF1(CT)  内脂素/内脏脂肪素(C端抗体)

hz-0270R PBEF(NT)  内脂素/内脏脂肪素抗体（N端）