神经细胞鸟苷酸置换因子NGEF抗体

英文名称    Ephexin-1    

中文名称    神经细胞鸟苷酸置换因子NGEF抗体    

别    名    ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.    

说 明 书    0.2ml      

研究领域    细胞生物  神经生物学  信号转导  G蛋白偶联受体  G蛋白信号      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,     

产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 神经细胞鸟苷酸置换因子NGEF抗体（石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    82kDa    

细胞定位    细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EPHEXIN (451-550aa)    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

神经细胞鸟苷酸置换因子NGEF抗体产品介绍    background:

EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.

Function:
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.

Subunit:
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase

Subcellular Location:
Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones.

Tissue Specificity:
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.

Post-translational modifications:
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.

Similarity:
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.    

神经细胞鸟苷酸置换因子NGEF抗体

hz-4022R phospho-PKA R2 (Ser96)  磷酸化蛋白激酶A受体2α亚基抗体

hz-3965R PKA regulatory subunit I beta  蛋白激酶受体相关1β抗体

hz-0115M PKB/AKT-1  蛋白激酶B

hz-3969R PRKAR1  蛋白激酶A调节亚基a1抗体

hz-3643R PLASTIN3/T Plastin  丝束蛋白T Plastin抗体

hz-3807R PLAP/Phospholipase A2 activator protein  磷脂酶A2激活蛋白抗体

hz-3534R PLC beta 3  磷酯酶Cβ3抗体

hz-3340R Phospho-PLC beta3 (Ser537)  磷酸化磷酯酶Cβ3抗体

hz-3341R Phospho-PLC beta3 (Ser1105)  磷酸化磷酯酶Cβ3抗体

hz-0267R PKC beta 1/2  蛋白激酶C beta 1/2抗体

hz-2505R phospho-PRKCZ(Thr500)  磷酸化蛋白激酶C(T500)抗体

hz-2718R phospho-PKC beta 1/2(Thr500)  磷酸化蛋白激酶C β1/β2(Thr500)抗体

hz-3333R Phospho-PKC alpha/beta II (Thr638/641)  磷酸化蛋白激酶C α/β2抗体

hz-3531R PKC alpha  蛋白激酶C α抗体

hz-3532R PLCG 2/PLC gamma 2  磷酯酶Cγ2抗体

hz-3338R Phospho-PLCG2(Tyr1217)  磷酸化磷酯酶Cγ2抗体

hz-3339R Phospho-PLC gamma 2 (Tyr759)  磷酸化磷酯酶Cγ2抗体

hz-3533R PLCG 1/PLC gamma 1  磷酯酶Cγ1抗体

hz-5547R Phospho-PLC gamma 2(Tyr753)  磷酸化磷酯酶Cγ2抗体

hz-3460R Phospho-PLC gamma 1 (Tyr771)  磷酸化磷酯酶Cγ1抗体

hz-3342R Phospho-PLC gamma 1(Ser1248)  磷酸化磷酯酶Cγ1抗体

hz-3343R Phospho-PLC γ1 (Tyr783)  磷酸化磷酯酶Cγ1抗体

hz-5546R Phospho-PLC gamma 1/PLCG1(Tyr775)  磷酸化磷酯酶Cγ1抗体

hz-5560R phospho-PRKCZ(Thr410)  磷酸化蛋白激酶C(T410)抗体

hz-2329R PKC epsilon  蛋白激酶C抗体

hz-3626R PKC-γ/SCA14  蛋白激酶C亚型G抗体

hz-3729R Phospho-PKC gamma (Thr514)  磷酸化蛋白激酶C亚型G抗体

hz-3730R Phospho-PKC gamma (Thr674)  磷酸化蛋白激酶C亚型G抗体

hz-3625R PKC delta  蛋白激酶C亚性D型抗体

hz-3726R phospho-PKC delta (Tyr52)  磷酸化蛋白激酶C亚性D型抗体

hz-3727R phospho-PKC delta (Thr505)  磷酸化蛋白激酶C亚性D型抗体

hz-3728R phospho-PKC delta (Tyr311)  磷酸化蛋白激酶C亚性D型抗体

hz-5565R phospho-PRKCB(Ser660)  磷酸化蛋白激酶C抗体