获取电话小鼠促甲状腺细胞胚胎发育因子(TEF)检测试剂盒说明上写封闭液用蔗糖溶液，一般是用BSA或酪蛋白的蛋白分子封闭空白位点，蔗糖封闭的原理：
1、小鼠促甲状腺细胞胚胎发育因子(TEF)检测试剂盒实验中，蔗糖本身没有封闭作用;
2、封闭液一般是BSA、酪蛋白等或者是Tween等。
3、加蔗糖的主要目的是保护ELISA板子吸附(包被)的蛋白，起到“保护剂”的作用。
4、蔗糖溶液一般在ELISA板子经过BSA封闭后加，当然也有将蔗糖加到封闭液中同时进行处理;那一种更好需要你实验后来验证。但多数选择前者。
封闭剂还可用5%的脱脂乳(市售即可)，0.4%的明胶，但是实验表明前者比较好，后者不容易洗干净未结合上的明胶。
小鼠促甲状腺细胞胚胎发育因子(TEF)检测试剂盒封闭剂的原理就是与酶联板上未结合抗原或抗体的微孔中用封闭剂封闭上，结合抗体或抗原的时候就不会产生非特异结合了，不会影响实验的准确度。
tween的作用：虽然不能单独封闭，但和BSA在封闭时会起到清洗非特异性结合或结合不牢固的蛋白的作用，假如抗体包被在板上会出现YY-
附件：小鼠促甲状腺细胞胚胎发育因子(TEF)检测试剂盒实验中缓冲溶液等试剂的配置
ELISA相关产品，详情见：封闭试剂、终止溶液、封闭肽、ELISA稀释液、ELISA试剂盒、ELISA缓冲液、ELISA洗脱缓冲液
为了保证小鼠促甲状腺细胞胚胎发育因子(TEF)检测试剂盒实验的准确性，实验操作中加入的缓冲液和各组分一定要精确，将洗涤液加入酶标板中的时候防止加入的试剂混入另外一个酶标孔，严防交叉污染。
HZ-EL-R0987c大鼠基质金属蛋白酶抑制因子4(TIMP-4)检测试剂盒Rat TIMP-4 (Tissue Inhibitors of Metalloproteinase 4) ELISA Kit
HZ-EL-R0988c大鼠组织多肽抗原(TPA)检测试剂盒Rat TPA (Tissue Polypeptide Antigen) ELISA Kit
HZ-EL-R0989c大鼠(SERP1)检测试剂盒Rat SERP1 (Stress-associated Endoplasmic Reticulum Protein 1) ELISA Kit
HZ-EL-R0990c大鼠Toll样受体4(TLR4)检测试剂盒Rat TLR4 (Toll-Like Receptor 4) ELISA Kit
HZ-EL-R0991c大鼠Toll样受体9(TLR9)检测试剂盒Rat TLR9 (Toll-Like Receptor 9) ELISA Kit
HZ-EL-R0995c大鼠c-myc癌基因产物(c-myc)检测试剂盒Rat c-myc (c-myc Oncogene Product) ELISA Kit
HZ-EL-R0996c大鼠多配体聚糖1(SDC1)检测试剂盒Rat SDC1 (Syndecan 1) ELISA Kit
HZ-EL-R0999c大鼠5脂加氧酶(5-LO)检测试剂盒 Rat 5-LO (Arachidonate 5-Lipoxygenase) ELISA Kit
HZ-EL-R1000c大鼠12脂加氧酶(12-LO)检测试剂盒 Rat 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit
HZ-EL-R1001c大鼠15脂加氧酶(15-LO)检测试剂盒 Rat 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit
HZ-EL-R1002c大鼠正五聚蛋白3(PTX3)检测试剂盒Rat PTX3 (Pentraxin 3) ELISA Kit
HZ-EL-R1003c大鼠20S-蛋白酶体(20S-PSM)检测试剂盒Rat 20S-PSM (20S-Proteasome) ELISA Kit
HZ-EL-R1004c大鼠细胞角蛋白18(CK-18/KRT18)检测试剂盒 Rat CK-18/KRT18 (Cytokeratin 18) ELISA Kit
HZ-EL-R1005c大鼠转录因子20(TCF20)检测试剂盒Rat TCF20 (Transcription factor 20) ELISA Kit
HZ-EL-R1006c大鼠雌激素受体相关受体α(ERRα)检测试剂盒Rat ERRα (Estrogen Related Receptor Alpha) ELISA Kit
HZ-EL-R1007c大鼠α2抗纤溶酶(α2-AP)检测试剂盒 Rat α2-AP (α2-Antiplasmin) ELISA Kit
HZ-EL-R1008c大鼠转铁蛋白受体蛋白2(TFR2)检测试剂盒Rat TFR2 (Transferrin Receptor Protein 2) ELISA Kit
HZ-EL-R1009c大鼠转铁蛋白受体(TFR/CD71)检测试剂盒Rat TFR (Transferrin Receptor) ELISA Kit
HZ-EL-R1010c大鼠白介素24 (IL-24)检测试剂盒Rat IL-24 (Interleukin 24) ELISA Kit
HZ-EL-R1011c大鼠转化生长因子受体β(TGF-βR)检测试剂盒Rat TGF-βR (Transforming Growth Factor Receptor) ELISA Kit
HZ-EL-R1012c大鼠转化生长因子α(TGF-α)检测试剂盒Rat TGF-α (Transforming Growth Factor Alpha) ELISA Kit
HZ-EL-R1014c大鼠血小板膜糖蛋白IV (GP4/CD36)检测试剂盒Rat GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit
HZ-EL-R1015c大鼠转化生长因子β2(TGF-β2)检测试剂盒Rat TGF-β2 (Transforming Growth Factor Beta 2) ELISA Kit
HZ-EL-R1016c大鼠转化生长因子β3(TGF-β3)检测试剂盒Rat TGF-β3 (Transforming Growth Factor Beta 3) ELISA Kit
HZ-EL-R1017c大鼠诱导转化生长因子β1(TGF βI)检测试剂盒Rat TGFβI (Transforming Growth Factor Beta Induced) ELISA Kit
HZ-EL-R1018c大鼠转化生长因子β刺激蛋白22(TSC22)检测试剂盒Rat TSC22 (Transforming Growth Factor Beta Stimulated Protein Clone 22) ELISA Kit
HZ-EL-R1019c大鼠谷氨酰胺转移酶(TG)检测试剂盒Rat TG (Transglutaminase) ELISA Kit
HZ-EL-R1020c大鼠跨膜emp 24域含有蛋白质1(TMED1)检测试剂盒Rat TMED1(Transmembrane Emp24 Domain Containing Protein 1) ELISA Kit
HZ-EL-R1021c大鼠转运蛋白1(TNPO1)检测试剂盒Rat TNPO1(Transportin 1) ELISA Kit
HZ-EL-R1022c大鼠甲状腺素转运蛋白(TTR)检测试剂盒Rat TTR (Transthyretin) ELISA Kit
HZ-EL-R1023c大鼠三叶因子2(TFF 2)检测试剂盒Rat TFF2 (Trefoil Factor 2) ELISA Kit
HZ-EL-R1024c大鼠肠三叶肽因子3(TFF3)检测试剂盒Rat TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit
HZ-EL-R1025c大鼠髓系细胞触发受体-1(TREM-1)检测试剂盒Rat TREM-1 (Triggering Receptor Expresses on Myeloid Cells-1) ELISA Kit