人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒

人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人垂体腺苷酸环化酶激活肽(ADCYAP1)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

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XY-EL-H2414c 人α-黑色素细胞刺激素(αMSH)ELISA试剂盒 Human αMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit

XY-EL-H2415c 人原卟啉(EP)ELISA试剂盒 Human EP (Erythrocyte Protoporphyrin) ELISA Kit

XY-EL-H2416c 人甲状腺素受体α(THRα)ELISA试剂盒 Human THRα (Thyroid Hormone Receptor Alpha) ELISA Kit

XY-EL-H2417c 人核因子相关κB结合蛋白 (NFRκB)ELISA试剂盒 Human NFRκB (Nuclear Factor Related To Kappa B Binding Protein) ELISA Kit

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XY-EL-H2419c 人端锚聚合酶2 (TNKS2)ELISA试剂盒 Human TNKS2 (Tankyrase 2) ELISA Kit

XY-EL-H2420c 人多聚ADP核糖聚合酶4(PARP4)ELISA试剂盒 Human PARP4 (Poly ADP Ribose Polymerase 4) ELISA Kit 

XY-EL-H2421c 人新蝶呤 (Neopterin)ELISA试剂盒 Human Neopterin ELISA Kit

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XY-EL-H2424c 人花生四烯酸-15-脂加氧酶B(ALOX15B)ELISA试剂盒 Human ALOX15B (Arachidonate-15-Lipoxygenase, Type B)ELISA Kit

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XY-EL-H2426c 人白介素24 (IL-24)ELISA试剂盒 Human IL-24 (Interleukin 24) ELISA Kit

XY-EL-H2427c 人黑素瘤缺乏因子2(AIM2)ELISA试剂盒 Human AIM2 (Absent In Melanoma 2) ELISA Kit

XY-EL-H2428c 人CD63/四旋蛋白30 (TSPAN30/CD63)ELISA试剂盒 Human TSPAN30/CD63 (Tetraspanin 30) ELISA Kit

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XY-EL-H2431c 人cGMP依赖性蛋白激酶I (PRKG1)ELISA试剂盒 Human PRKG1 (Protein Kinase, cGMP Dependent Type I) ELISA Kit

XY-EL-H2432c 人磷酸二酯酶4D(PDE4D)ELISA试剂盒 Human PDE4D (Phosphodiesterase 4D, cAMP Specific) ELISA Kit

XY-EL-H2433c 人脑型肌酸激酶(CKB)ELISA试剂盒 Human CKB (Creatine Kinase, Brain) ELISA Kit

XY-EL-H2434c 人抗肝肾微粒体抗体(Anti-LKM-1 IgG)ELISA试剂盒 Human Anti-LKM-1 IgG(Anti-Liver-Kidney Microsomes-1 IgG) ELISA Kit

XY-EL-H2435c 人赖氨酰氧化酶样蛋白4(LOXL4)ELISA试剂盒 Human LOXL4 (Lysyl Oxidase Like Protein 4 ) ELISA Kit

XY-EL-H2436c 人肿瘤坏死因子受体超家族成员1B(TNFRSF1B)ELISA试剂盒 Human TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit

XY-EL-H2437c 人抗环胍氨酸肽抗体(ACCPA)ELISA试剂盒 Human ACCPA (anti-cyclic citrullinated peptide antibody) ELISA Kit

XY-EL-H2438c 人杀菌肽LL-37(LL-37)ELISA试剂盒 Human LL-37 (Antibacterial Protein LL-37) ELISA Kit

XY-EL-H2439c 人胸腺肽α1(Tα1)ELISA试剂盒 Human Tα1 (Thymosin-α1) ELISA Kit