人血红素氧合酶1(HO1)ELISA试剂盒

人血红素氧合酶1(HO1)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人血红素氧合酶1(HO1)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人血红素氧合酶1(HO1)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人血红素氧合酶1(HO1)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人血红素氧合酶1(HO1)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1523c 人巨噬细胞刺激蛋白(MSP)ELISA试剂盒 Human MSP (Macrophage Stimulating Protein) ELISA Kit

XY-EL-H1524c 人巨噬细胞活化因子(MAF)ELISA试剂盒 Human MAF (Macrophage Activating Factor) ELISA Kit

XY-EL-H1525c 人巨噬细胞集落刺激因子受体(MCSFR)ELISA试剂盒 Human MCSFR (Macrophage Colony Stimulating Factor Receptor) ELISA Kit

XY-EL-H1526c 人巨噬细胞趋化因子(MCF)ELISA试剂盒 Human MCF (Macrophage Chemotatic Factor) ELISA Kit

XY-EL-H1528c 人巨噬细胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA试剂盒 Human MIP-3β/ELC/CCL19 (Macrophage Inflammatory Protein 3β) ELISA Kit 

XY-EL-H1529c 人巨噬细胞炎性蛋白5(MIP-5)ELISA试剂盒 Human MIP-5 (Macrophage Inflammatory Protein 5) ELISA Kit 

XY-EL-H1530c 人巨噬细胞移动抑制因子(MIF)ELISA试剂盒 Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit

XY-EL-H1531c 人卷曲同源物8(FZD8)ELISA试剂盒 Human FZD8 (Frizzled Homolog 8) ELISA Kit

XY-EL-H1534c 人细胞程序性死亡蛋白1(PDCD1)ELISA试剂盒 Human PDCD1 (Programmed Cell Death Protein 1) ELISA Kit

XY-EL-H1535c 人别孕烯醇酮(AP)ELISA试剂盒 Human AP (Allopregnanolone) ELISA Kit

XY-EL-H1536c 人促肾上腺皮质激素释放激素结合蛋白(CRHBP)ELISA试剂盒   Human CRHBP (Corticotropin Releasing Hormone Bingding Protein) ELISA Kit

XY-EL-H1537c 人α-血红蛋白稳定蛋白(αHSP)ELISA试剂盒   Human αHSP (Alpha-Hemoglobin Stabilizing Protein) ELISA Kit

XY-EL-H1538c 人基质金属蛋白酶28(MMP-28)ELISA试剂盒 Human MMP-28 (Matrix Metalloproteinase 28) ELISA Kit

XY-EL-H1539c 人Toll样受体4(TLR4)ELISA试剂盒 Human TLR4 (Toll-Like Receptor 4) ELISA Kit

XY-EL-H1540c 人α-胞衬蛋白(SPTAN1)ELISA试剂盒 Human SPTAN1 (Alpha-Fodrin) ELISA Kit 

XY-EL-H1541c 人锌结合α2-糖蛋白1(AZGP1)ELISA试剂盒 Human AZGP1 (Zinc-alpha-2-glycoprotein) ELISA Kit

XY-EL-H1542c 人抗促甲状腺素受体抗体(TSHRAb)ELISA试剂盒 Human TSHRAb (Thyroid Stimulating Hormone Receptor Antidoby) ELISA Kit

XY-EL-H1543c 人间皮素(MSLN)ELISA试剂盒 Human  MSLN (Mesothelin) ELISA Kit

XY-EL-H1544c 人硬骨素(SOST)ELISA试剂盒 Human SOST (Sclerostin) ELISA Kit

XY-EL-H1545c 人干扰素调节因子5(IRF5)ELISA试剂盒 Human IRF5 (Interferon Regulatory Factor 5) ELISA Kit

XY-EL-H1546c 人沉默调节蛋白1(SIRT1)ELISA试剂盒 Human SIRT1 (Sirtuin 1) ELISA Kit

XY-EL-H1547c 人细胞程序性死亡蛋白1配体1(PDCD1LG1)ELISA试剂盒 Human PDCD1LG1 (Programmed Cell Death Protein 1 Ligand 1) ELISA Kit

XY-EL-H1548c 人抗肝特异性脂蛋白(LSP)ELISA试剂盒 Human LSP (Liver Specific Lipoprotein) ELISA Kit

XY-EL-H1549c 人抗核膜糖蛋白210抗体(AGPA)ELISA试剂盒   Human AGPA (Anti-Glucoprotein 210 Antibody) ELISA Kit

XY-EL-H1550c 人τ-蛋白激酶1(τPK1)ELISA试剂盒 Human τPK1 (Tau-Protein Kinase) ELISA Kit

XY-EL-H1551c 人抗酒石酸酸性磷酸酶(ACP5)ELISA试剂盒 Human ACP5 (Tartrate Resistant Acid Phosphatase 5) ELISA Kit

XY-EL-H1552c 人血管性血友病因子裂解蛋白酶(VWFCP/ADAMTS13)ELISA试剂盒 Human VWFCP/ADAMTS13 (Von Willebrand Factor Cleaving Protease) ELISA Kit

XY-EL-H1553c 人5-甲基四氢叶酸高半胱氨酸甲基转移酶(MTR)ELISA试剂盒 Human MTR (5-Methyltetrahydrofolate Homocysteine Methyltransferase) ELISA Kit

XY-EL-H1554c 人高迁移率族蛋白B1(HMGB-1)ELISA试剂盒 Human HMGB-1 (High mobility group protein B1) ELISA Kit

XY-EL-H1555c 人胎盘生长因子(PGF)ELISA试剂盒 Human PGF (Placental Growth Factor) ELISA Kit

XY-EL-H1556c 人叉头框蛋白C2(FOXC2)ELISA试剂盒 Human FOXC2 (Forkhead Box Protein C2) ELISA Kit

XY-EL-H1557c 人内皮细胞特异分子1(ESM1)ELISA试剂盒 Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit

XY-EL-H1558c 人Ras相关C3肉毒菌毒素底物1(Rac1)ELISA试剂盒 Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit

XY-EL-H1559c 人抗生长激素抗体(GHAb)ELISA试剂盒 Human GHAb (Anti-Growth Hormone Antibody) ELISA Kit

XY-EL-H1560c 人白细胞免疫球蛋白样受体亚家族B成员2(LILRB2)ELISA试剂盒 Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2) ELISA Kit

XY-EL-H1561c 人催产素受体(OTR)ELISA试剂盒 Human OTR (Oxytocin Receptor) ELISA Kit