人食欲素A(OXA)ELISA试剂盒

人食欲素A(OXA)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人食欲素A(OXA)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人食欲素A(OXA)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人食欲素A(OXA)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人食欲素A(OXA)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1300c 人干细胞因子受体(SCFR/c-Kit)ELISA试剂盒 Human SCFR/c-Kit (Stem Cell Factor Receptor) ELISA Kit 

XY-EL-H1301c 人甘丙肽/甘丙素(GAL)ELISA试剂盒 Human GAL (Galanin) ELISA Kit

XY-EL-H1302c 人甘丙肽/甘丙素受体1(GALR1)ELISA试剂盒 Human GALR1 (Galanin Receptor 1) ELISA Kit 

XY-EL-H1303c 人甘露聚糖结合凝集素相关丝氨酸蛋白酶(MASP)ELISA试剂盒 Human MASP (Mannan Binding Lectin Associated Serine Protease) ELISA Kit

XY-EL-H1305c 人甘露糖结合蛋白(MBP/MBL)ELISA试剂盒 Human MBP/MBL (Mannose Binding Protein/Mannose Binding Lectin) ELISA Kit

XY-EL-H1308c 人磷酸甘油酸变位酶1(PGAM1)ELISA试剂盒 Human PGAM1 (Phosphoglycerate Mutase 1) ELISA Kit

XY-EL-H1309c 人肝癌抗原(PHC)ELISA试剂盒 Human PHC (Primary Hepatic Carcinoma)ELISA Kit

XY-EL-H1310c 人肝脏/红细胞丙酮酸激酶(PK)ELISA试剂盒 Human PK (Pyruvate Kinase, Liver And RBC) ELISA Kit 

XY-EL-H1311c 人肝脏糖原磷酸化酶(PYGL)ELISA试剂盒 Human PYGL (Glycogen Phosphorylase, Liver) ELISA Kit 

XY-EL-H1312c 人分泌型卷曲相关蛋白2 (SFRP2)ELISA试剂盒 Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit

XY-EL-H1313c 人高尔基蛋白73(GP-73)ELISA试剂盒 Human GP-73 (Golgi Protein 73) ELISA Kit

XY-EL-H1314c 人高分子量细胞角蛋白(CK-HMW)ELISA试剂盒 Human CK-HMW (Cytokeratin High Molecular Weight) ELISA Kit

XY-EL-H1315c 人唾液酸乙酰酯酶(SIAE)ELISA试剂盒 Human SIAE (Sialic Acid Acetylesterase) ELISA Kit

XY-EL-H1316c 人同线蛋白2(ST2)ELISA试剂盒 Human ST2 (Syntenin 2) ELISA Kit 

XY-EL-H1317c 人唾液酸结合免疫球蛋白样凝集素1(SIGLEC1)ELISA试剂盒 Human SIGLEC1 (Sialic Acid Binding Ig Like Lectin 1) ELISA Kit

XY-EL-H1318c 人高铁血红蛋白(MHB)ELISA试剂盒 Human MHB (Methemoglobin) ELISA Kit

XY-EL-H1319c 人睾酮受体(TR)ELISA试剂盒 Human TR (Testosterone Receptor) ELISA Kit

XY-EL-H1320c 人唾液酸结合免疫球蛋白样凝集素10(SIGLEC10)ELISA试剂盒 Human SIGLEC10 (Sialic Acid Binding Immunoglobulin Like Lectin 10) ELISA Kit

XY-EL-H1321c 人唾液酸结合免疫球蛋白样凝集素2(SIGLEC2)ELISA试剂盒 Human SIGLEC2 (Sialic Acid Binding Immunoglobulin Like Lectin 2) ELISA Kit

XY-EL-H1322c 人肽基精氨酸脱亚氨酶IV(PADI4)ELISA试剂盒 Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit

XY-EL-H1323c 人唾液酸结合免疫球蛋白样凝集素8(SIGLEC8)ELISA试剂盒 Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) ELISA Kit

XY-EL-H1324c 人Smad同源物3 (Smad3/MADH3)ELISA试剂盒 Human Smad3/MADH3 (Mothers Against Decapentaplegic Homolog 3) ELISA Kit

XY-EL-H1325c 人孤腓肽(OFQ/N)ELISA试剂盒 Human OFQ/N (Orphanin FQ/Nociceptin) ELISA Kit

XY-EL-H1326c 人谷氨酸半胱氨酸连接酶修饰亚基(GCLM)ELISA试剂盒 Human GCLM (Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit 

XY-EL-H1327c 人谷氨酸脱氢酶(GDH/GLDH)ELISA试剂盒   Human GDH/GLDH (Glutamate dehydrogenase) ELISA Kit

XY-EL-H1328c 人谷氨酸脱羧酶(GAD)ELISA试剂盒 Human GAD (Glutamic Acid Decarboxylase) ELISA Kit

XY-EL-H1329c 人谷氨酸脱羧酶2(GAD2)ELISA试剂盒 Human GAD2 (Glutamate Decarboxylase 2) ELISA Kit 

XY-EL-H1330c 人谷氨酸脱羧酶抗体(GAD-Ab/Anti-GAD)ELISA试剂盒 Human GAD-Ab (Anti-Glutamic Acid Decarboxylase antibody) ELISA Kit

XY-EL-H1332c 人谷胱甘肽S转移酶(GSTs)ELISA试剂盒 Human GSTs (Glutathione S-Transferases) ELISA Kit

XY-EL-H1333c 人谷胱甘肽S-转移酶α1(GSTα1)ELISA试剂盒 Human GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit 

XY-EL-H1334c 人谷胱甘肽S-转移酶θ1(GSTθ1/GSTt1)ELISA试剂盒 Human GSTθ1/GSTt1 (Glutathione S Transferase Theta 1) ELISA Kit 

XY-EL-H1335c 人谷胱甘肽S转移酶θ2(GSTθ2/GSTt2)ELISA试剂盒 Human GSTθ2/GSTt2 (Glutathione S Transferase Theta 2) ELISA Kit

XY-EL-H1336c 人谷胱甘肽S转移酶ω1(GSTω1)ELISA试剂盒 Human GSTω1 (Glutathione S Transferase Omega 1) ELISA Kit 

XY-EL-H1337c 人组蛋白脱乙酰基酶(HDAC2)ELISA试剂盒 Human HDAC2 (Histone Deacetylase 2) ELISA Kit

XY-EL-H1338c 人C1q肿瘤坏死因子相关蛋白1(C1QTNF1)ELISA试剂盒 Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) ELISA Kit

XY-EL-H1339c 人谷胱甘肽还原酶(GR)ELISA试剂盒 Human GR (Glutathione Reductase) ELISA Kit 

XY-EL-H1340c 人谷胱甘肽S转移酶pi基因(GSTpi)ELISA试剂盒 Human GSTpi (Glutathione S Transferases Pi) ELISA Kit

XY-EL-H1341c 人骨保护素(OPG)ELISA试剂盒 Human OPG (Osteoprotegerin) ELISA Kit 

XY-EL-H1342c 人骨保护素配体(OPGL)ELISA试剂盒 Human OPGL (Osteoprotegerin Ligand) ELISA Kit

XY-EL-H1343c 人骨钙素(OC/BGP)ELISA试剂盒 Human OC/BGP (Osteocalcin) ELISA Kit

XY-EL-H1344c 人弹性蛋白酶3B(ELA3B)ELISA试剂盒   Human ELA3B (Elastase 3B) ELISA Kit

XY-EL-H1345c 人骨骼肌特异性受体酪氨酸激酶(MUSK)ELISA试剂盒 Human MUSK (Muscle Skeletal Receptor Tyrosine Kinase) ELISA Kit

XY-EL-H1346c 人谷胱甘肽S转移酶α5(GSTα5)ELISA试剂盒 Human GSTα5 (Glutathione S Transferase Alpha 5) ELISA Kit 

XY-EL-H1347c 人骨桥素(OPN)ELISA试剂盒 Human OPN (Osteopontin) ELISA Kit