人脑红蛋白(NGB)ELISA试剂盒

人脑红蛋白(NGB)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人脑红蛋白(NGB)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人脑红蛋白(NGB)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人脑红蛋白(NGB)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人脑红蛋白(NGB)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H1264c 人肺癌标志物DR-70(DR-70TM)ELISA试剂盒 Human DR-70TM (Tumor Marker DR-70 For Lung Cancer) ELISA Kit

XY-EL-H1265c 人胰腺再生蛋白1β(REG1β)ELISA试剂盒 Human REG1β (Regenerating Islet Derived Protein 1 Beta) ELISA Kit 

XY-EL-H1266c 人肺表面活性物质相关蛋白A(SPA)ELISA试剂盒 Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit 

XY-EL-H1267c 人肺表面活性物质相关蛋白B(SPB)ELISA试剂盒 Human SPB (Pulmonary Surfactant Associated Protein B) ELISA Kit 

XY-EL-H1268c 人肺表面活性物质相关蛋白C(SPC)ELISA试剂盒 Human SPC (Pulmonary Surfactant Associated Protein C) ELISA Kit 

XY-EL-H1269c 人肺表面活性物质相关蛋白D(SPD)ELISA试剂盒 Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit 

XY-EL-H1270c 人肺部活化调节趋化因子(PARC/CCL18)ELISA试剂盒 Human PARC/CCL18 (Pulmonary Activation Regulated Chemokine) ELISA Kit 

XY-EL-H1271c 人肺耐药蛋白(LRP)ELISA试剂盒 Human LRP (Lung Resistance-Related Protein) ELISA Kit

XY-EL-H1272c 人丝氨酸蛋白酶8(PRSS8)ELISA试剂盒 Human PRSS8 (Protease, Serine 8) ELISA Kit 

XY-EL-H1273c 人SPARC样蛋白1(SPARCL1)ELISA试剂盒 Human SPARCL1 (SPARC Like Protein 1) ELISA Kit

XY-EL-H1274c 人信号素3A(SEMA3A)ELISA试剂盒 Human SEMA3A (Semaphorin 3A) ELISA Kit

XY-EL-H1275c 人分泌型免疫球蛋白A(sIgA)ELISA试剂盒 Human sIgA (Secretory Immunoglobulin A) ELISA Kit 

XY-EL-H1276c 人分泌性白细胞蛋白酶抑制因子(SLPI)ELISA试剂盒 Human SLPI (Secretory Leukocyte Protease Inhibitor) ELISA Kit

XY-EL-H1277c 人低氧诱导因子1α (HIF-1α)ELISA试剂盒 Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit

XY-EL-H1278c 人血清淀粉样蛋白A3(SAA3)ELISA试剂盒 Human SAA3 (Serum Amyloid A3) ELISA Kit

XY-EL-H1279c 人血清淀粉样蛋白P(SAP)ELISA试剂盒 Human SAP (Serum Amyloid P Component) ELISA Kit

XY-EL-H1280c 人蛋白酶激活受体4(PAR4)ELISA试剂盒 Human PAR4 (Protease Activated Receptor 4) ELISA Kit 

XY-EL-H1281c 人脯氨酰羧肽酶(PRCP)ELISA试剂盒 Human PRCP (Prolylcarboxypeptidase) ELISA Kit 

XY-EL-H1282c 人复制蛋白A(RPA-70)ELISA试剂盒 Human RPA-70 (replication protein A) ELISA Kit

XY-EL-H1283c 人G蛋白偶联受体120(GPR120)ELISA试剂盒 Human GPR120 (G Protein Coupled Receptor 120) ELISA Kit

XY-EL-H1284c 人胰腺再生蛋白3α(REG3α)ELISA试剂盒 Human REG3α (Regenerating Islet Derived Protein 3 Alpha) ELISA Kit 

XY-EL-H1285c 人富含半胱氨酸蛋白61(Cyr61)ELISA试剂盒 Human Cyr61 (Cysteine Rich Protein, Angiogenic Inducer 61) ELISA Kit

XY-EL-H1286c 人富含半胱氨酸型酸性蛋白(SPARC)ELISA试剂盒 Human SPARC (Secreted Protein Acidic And Rich In Cysteine) ELISA Kit

XY-EL-H1287c 人亮氨酸丰富α2糖蛋白1(LRG1)ELISA试剂盒 Human LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit

XY-EL-H1288c 人胰腺再生蛋白3γ(REG3γ)ELISA试剂盒 Human REG3γ (Regenerating Islet Derived Protein 3 Gamma) ELISA Kit 

XY-EL-H1289c 人S100钙结合蛋白A8(S100A8)ELISA试剂盒 Human S100A8 (S100 Calcium Binding Protein A8) ELISA Kit

XY-EL-H1290c 人S100钙结合蛋白A9(S100A9)ELISA试剂盒 Human S100A9 (S100 Calcium Binding Protein A9) ELISA Kit

XY-EL-H1291c 人G蛋白偶联受体37(GPR37)ELISA试剂盒 Human GPR37 (G Protein Coupled Receptor 37) ELISA Kit

XY-EL-H1292c 人S100钙结合蛋白A11(S100A11)ELISA试剂盒 Human S100A11 (S100 Calcium Binding Protein A11) ELISA Kit  

XY-EL-H1293c 人S100钙结合蛋白A12(S100A12 )ELISA试剂盒 Human S100A12 (S100 Calcium Binding Protein A12) ELISA Kit

XY-EL-H1294c 人S100钙结合蛋白A13(S100A13 )ELISA试剂盒 Human S100A13 (S100 Calcium Binding Protein A13) ELISA Kit

XY-EL-H1295c 人S100钙结合蛋白A6(S100A6)ELISA试剂盒 Human S100A6 (S100 Calcium Binding Protein A6) ELISA Kit 

XY-EL-H1296c 人S100钙结合蛋白A7 (S100A7)ELISA试剂盒 Human S100A7 (S100 Calcium Binding Protein A7) ELISA Kit

XY-EL-H1297c 人S100钙结合蛋白B (S100B)ELISA试剂盒 Human S100B (S100 Calcium Binding Protein B) ELISA Kit 

XY-EL-H1298c 人多配体聚糖1(SDC1)ELISA试剂盒 Human SDC1 (Syndecan 1) ELISA Kit 

XY-EL-H1299c 人干细胞因子(SCF)ELISA试剂盒 Human SCF (Stem Cell Factor) ELISA Kit