人水通道蛋白9(AQP9)ELISA试剂盒

人水通道蛋白9(AQP9)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人水通道蛋白9(AQP9)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人水通道蛋白9(AQP9)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人水通道蛋白9(AQP9)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人水通道蛋白9(AQP9)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H0914c 人胎盘钙黏蛋白(P-Cadherin)ELISA试剂盒 Human P-Cadherin (Cadherin, Placental) ELISA Kit

XY-EL-H0915c 人P糖蛋白/渗透性糖蛋白(P-GP)ELISA试剂盒 Human P-GP (Permeability Glycoprotein) ELISA Kit

XY-EL-H0917c 人P选择素(SELP)ELISA试剂盒 Human SELP (P-Selectin) ELISA Kit 

XY-EL-H0918c 人Rap鸟嘌呤交换因子1(RAPGEF1)ELISA试剂盒 Human RAPGEF1 (Rap Guanine Nucleotide Exchange Factor 1) ELISA Kit 

XY-EL-H0919c 人Rac-GTP酶激活蛋白1(Rac-GAP1)ELISA试剂盒 Human Rac-GAP1 (Rac-GTPase Activating Protein 1) ELISA Kit 

XY-EL-H0920c 人ras同源物基因家族成员b(RHOB)ELISA试剂盒 Human RHOB (Ras Homolog Gene Family, Memmber B) ELISA Kit

XY-EL-H0921c 人REST协阻抑因子3(RCOR3)ELISA试剂盒 Human RCOR3 (Rest Corepressor 3) ELISA Kit

XY-EL-H0922c 人ras同源物基因家族成员a(RHOA)ELISA试剂盒 Human RHOA (Ras Homolog Gene Family, Memmber A) ELISA Kit

XY-EL-H0923c 人Rho关联卷曲螺旋蛋白激酶2(Rock-2)ELISA试剂盒 Human Rock-2 (Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit

XY-EL-H0924c 人Rho家族GTP酶1(RND1)ELISA试剂盒 Human RND1 (Rho Family GTPase 1) ELISA Kit

XY-EL-H0925c 人Runt相关转录因子2(RUNX2)ELISA试剂盒 Human RUNX2 (Runt-Related Transcription Factor 2) ELISA Kit

XY-EL-H0926c 人IX型胶原α3 (COL9α3)ELISA试剂盒 Human COL9α3 (Collagen Type IX Alpha 3) ELISA Kit

XY-EL-H0927c 人S100蛋白(S-100)ELISA试剂盒 Human S-100 (Soluble Protein-100) ELISA Kit

XY-EL-H0928c 人Salusin α蛋白(Salusin α)ELISA试剂盒 Human Salusin α (Salusin Alpha) ELISA Kit

XY-EL-H0929c 人SH2携带蛋白(SHC/SLP-76)ELISA试剂盒 Human SHC/SLP-76 (SH2-Containing Protein) ELISA Kit

XY-EL-H0930c 人音猬因子(SHH )ELISA试剂盒 Human SHH (Hedgehog Homolog, Sonic) ELISA Kit 

XY-EL-H0931c 人Slit同源物2(Slit2)ELISA试剂盒 Human Slit2 (Slit Homolog 2) ELISA Kit

XY-EL-H0932c 人Smad同源物1 (Smad1/MADH1)ELISA试剂盒 Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit

XY-EL-H0933c 人Smad同源物4 (Smad4/MADH4)ELISA试剂盒 Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4 )ELISA Kit

XY-EL-H0934c 人Smad同源物7 (Smad7/MADH7)ELISA试剂盒 Human Smad7/MADH7 (Mothers Against Decapentaplegic Homolog 7) ELISA Kit

XY-EL-H0935c 人Sp100核抗原(Sp100)ELISA试剂盒 Human Sp100 (Sp100 Nuclear Antigen) ELISA Kit 

XY-EL-H0936c 人STAT蛋白抑制因子1(PIAS1)ELISA试剂盒 Human PIAS1 (Protein Inhibitor of Activated STAT 1) ELISA Kit

XY-EL-H0937c 人STAT蛋白抑制因子3(PIAS3)ELISA试剂盒 Human PIAS3 (Protein Inhibitor of Activated STAT3) ELISA Kit

XY-EL-H0938c 人原钙黏素β16(PCDHβ16)ELISA试剂盒 Human PCDHβ16 (Protocadherin Beta 16) ELISA Kit 

XY-EL-H0939c 人V型胶原α2(COL5α2)ELISA试剂盒 Human COL5α2 (Collagen Type V Alpha 2) ELISA Kit  

XY-EL-H0940c 人S抗原(SAG)ELISA试剂盒 Human SAG (S Antigen) ELISA Kit 

XY-EL-H0941c 人TH糖蛋白(THP)ELISA试剂盒 Human THP (Tamm–Horsfall Glycoprotein) ELISA Kit

XY-EL-H0942c 人43KDa Tar DNA结合蛋白(TDP43)ELISA试剂盒 Human TDP43 (Tar DNA Binding Protein 43KDa )ELISA Kit 

XY-EL-H0943c 人TATA结合蛋白(TBP)ELISA试剂盒 Human TBP (TATA Binding Protein) ELISA Kit 

XY-EL-H0944c 人TATA框结合蛋白/TBP相关因子(TAF)ELISA试剂盒 Human TAF (TATA Box Binding Protein/TBP-Associated Factors) ELISA Kit

XY-EL-H0945c 人TATA框结合蛋白相关因子1(TAF1)ELISA试剂盒 Human TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit 

XY-EL-H0946c 人TATA框结合蛋白相关因子2(TAF2)ELISA试剂盒 Human TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit

XY-EL-H0947c 人TATA框结合蛋白相关因子13(TAF13)ELISA试剂盒 Human TAF13 (TATA Box Binding Protein Associated Factor 13) ELISA Kit

XY-EL-H0948c 人微管关联蛋白(MAPτ)ELISA试剂盒 Human MAPτ (Microtubule Associated Protein Tau/Tau Protein) ELISA Kit

XY-EL-H0949c 人TGF-β诱导早期应答基因1(TIEG1)ELISA试剂盒 Human TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA Kit

XY-EL-H0951c 人Toll样受体2(TLR2)ELISA试剂盒 Human TLR2 (Toll-Like Receptor 2) ELISA Kit

XY-EL-H0952c 人Toll样受体9(TLR9)ELISA试剂盒 Human TLR9 (Toll-Like Receptor 9) ELISA Kit