人抑制素A(INHA)ELISA试剂盒

人抑制素A(INHA)ELISA试剂盒原理

辣根过氧化物酶 (horse radish peroxidase，简称HRP，EC.1.11.1.7)是植物中研究得最深入的一种过氧化物酶，早在20世纪30年代就有人着手从辣根中分离此酶，以后又制备出结晶。本实验是以辣根为原料，经过水的抽提，硫酸铵和丙酮分级分离，再经锌离子纯化，透析除盐，冰冻干燥，便可得到高纯度的辣根过氧化物酶。

人抑制素A(INHA)ELISA试剂盒辣根过氧化物酶是一种含亚铁血红素的蛋白质，HRP广泛分布于植物界，它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白，糖含量18%。HRP由多个同功酶组成，Mr在40000左右，等电点7.2。溶于水，溶解度为5%(W/V)，溶液呈棕红色，透明。人抑制素A(INHA)ELISA试剂盒酶催化的最适PH因供氢体不同而稍有差异，但多在pH5左右。酶溶于水和58%以下的硫酸铵溶液。HRP可溶于0.58饱和度以下的硫酸铵溶液，而0.62饱和度以上则不溶。该酶最适pH7.0(对愈创木酚为氢给体而言)。在室温下，HRP几周内稳定，加热到63℃，在15min内稳定。人抑制素A(INHA)ELISA试剂盒氧化还原电势很低，pH6.08时，E 0 =-0.2070V，pH为7.71时，E′0 =-0.2787V。HRP的辅基和酶蛋白最大吸收光谱分别为403nm和275nm，一般以OD403nm/OD275nm的比值RZ(德文Reinheit Zahl)表示酶的纯度。* ?# p. n2 ~1 w. X3 b

酶标记物包括酶标记抗原、酶标记抗体和酶标记SPA等。酶标记物质量的好坏直接关系到免疫酶技术的成功与否，因此被称为关键的试剂。酶标记物中最常用的是酶标记抗体，它是将酶与特异性抗体经适当方法连接而成。酶标记抗体的质量主要取决于纯度好、活性强及亲和力高的酶和抗体，其次要有良好的制备方法。目前，高质量的酶(如辣根过氧化物酶，简称HRP)国内已有商品供应。人抑制素A(INHA)ELISA试剂盒高质量的抗体则可通过提取纯化而获得。在制备方法上，宜选用产率高、不影响结合物的活性和不混杂干扰性物质且操作简便易行的方法。

XY-EL-H0836c 人I型胶原交联氨基端肽(NTXI)ELISA试剂盒   Human NTXI (Cross Linked N-telopeptide of Type I Collagen) ELISA Kit

XY-EL-H0837c 人II型胶原交联羧基端肽(CTX-II)ELISA试剂盒 Human CTX-II (Cross Linked C-telopeptide of Type II Collagen) ELISA Kit

XY-EL-H0838c 人乳酸脱氢酶B(LDHB)ELISA试剂盒   Human LDHB (Lactate Dehydrogenase B) ELISA Kit 

XY-EL-H0839c 人Ⅱ型胶原螺旋肽(HELIX-Ⅱ)ELISA试剂盒 Human HELIX-Ⅱ(Type Ⅱ Collagen Helical Peptide) ELISA Kit

XY-EL-H0840c 人3-羟基-3-甲基戊二酰辅酶A合酶1(HMGCS1)ELISA试剂盒 Human HMGCS1 (3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1) ELISA Kit

XY-EL-H0841c 人Ⅲ型前胶原羧基端原肽(PⅢCP)ELISA试剂盒 Human PⅢCP (Procollagen Ⅲ C-Terminal ProPeptide) ELISA Kit 

XY-EL-H0842c 人Dickkopf 相关蛋白4(DKK4)ELISA试剂盒 Human DKK4 (Dickkopf Related Protein 4) ELISA Kit 

XY-EL-H0843c 人乳酸脱氢酶(LDH)ELISA试剂盒   Human LDH (Lactate Dehydrogenase) ELISA Kit 

XY-EL-H0844c 人亚甲基四氢叶酸还原酶(MTHFR)ELISA试剂盒 Human MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

XY-EL-H0845c 人AMP活化蛋白激酶α1(PRKAα1)ELISA试剂盒 Human PRKAα1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit 

XY-EL-H0846c 人B细胞κ 轻肽基因增强子核因子抑制因子ε(Nfkbie)ELISA试剂盒 Human NFKBIE (Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-cells Inhibitor Epsilon) ELISA Kit

XY-EL-H0847c 人蛋白酪氨酸磷酸酶受体B(PTPRB)ELISA试剂盒 Human PTPRB (Protein Tyrosine Phosphatase Receptor Type B ) ELISA Kit 

XY-EL-H0848c 人B因子(BF)ELISA试剂盒   Human BF (Factor B) ELISA Kit

XY-EL-H0849c 人cAMP反应元件结合蛋白(CREB)ELISA试剂盒 Human CREB (Cyclic AMP Response Element Binding Protein) ELISA Kit

XY-EL-H0851c 人和肽素(CPP)ELISA试剂盒 Human CPP (Copeptin) ELISA Kit

XY-EL-H0852c 人封闭蛋白11(CLDN11)ELISA试剂盒 Human CLDN11 (Claudin 11) ELISA Kit 

XY-EL-H0853c 人CXC趋化因子受体1(CXCR1)ELISA试剂盒 Human CXCR1 (CXC-Chemokine Receptor 1) ELISA Kit

XY-EL-H0854c 人CXC趋化因子受体3(CXCR3)ELISA试剂盒 Human CXCR3 (Chemokine CXC Receptor 3) ELISA Kit

XY-EL-H0855c 人分泌粒蛋白II (SCG2)ELISA试剂盒 Human SCG2 (Secretogranin II) ELISA Kit

XY-EL-H0856c 人C-Myc结合蛋白(MYCBP)ELISA试剂盒 Human MYCBP (C-Myc Binding Protein) ELISA Kit

XY-EL-H0857c 人V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)ELISA试剂盒 Human MYC (V-Myc Myelocytomatosis Viral Oncogene Homolog) ELISA Kit

XY-EL-H0858c 人C型凝集素结构域家族2成员C(CLEC2C)ELISA试剂盒 Human CLEC2C (C-Type Lectin Domain Family 2 Member C) ELISA Kit 

XY-EL-H0859c 人C型凝集素结构域家族4成员E(CLEC4E)ELISA试剂盒 Human CLEC4E (C-Type Lectin Domain Family 4 Member E) ELISA Kit

XY-EL-H0860c 人乳酸脱氢酶C(LDHC)ELISA试剂盒   Human LDHC (Lactate Dehydrogenase C) ELISA Kit 

XY-EL-H0861c 人酪氨酸蛋白激酶受体A2(EphA2)ELISA试剂盒 Human EphA2 (Tyrosine protein kinase receptor A2) ELISA Kit

XY-EL-H0862c 人Dickkopf 相关蛋白2(DKK2)ELISA试剂盒 Human DKK2 (Dickkopf Related Protein 2) ELISA Kit 

XY-EL-H0863c 人DNA结合蛋白(DBP)ELISA试剂盒 Human DBP (DNA Binding Protein) ELISA Kit

XY-EL-H0864c 人D-二聚体(D2D)ELISA试剂盒 Human D2D (D-Dimer) ELISA Kit

XY-EL-H0866c 人D-乳酸脱氢酶(D-LDH)ELISA试剂盒   Human D-LDH (D-Lactate Dehydrogenase) ELISA Kit 

XY-EL-H0867c 人凝血因子Ⅷ(F8)ELISA试剂盒   Human F8 (Coagulation Factor Ⅷ) ELISA Kit

XY-EL-H0868c 人增殖相关蛋白2G4 (PA2G4)ELISA试剂盒 Human PA2G4 (Proliferation Associated Protein 2G4) ELISA Kit

XY-EL-H0869c 人Ⅰ型胶原α1(COL1α1)ELISA试剂盒 Human COL1α1 (Collagen Type I Alpha 1) ELISA Kit  

XY-EL-H0870c 人VI型胶原α3(COL6α3)ELISA试剂盒 Human COL6α3 (Collagen Type VI Alpha 3) ELISA Kit 

XY-EL-H0871c 人XIII型胶原 (COL13)ELISA试剂盒 Human COL13 (Collagen Type XIII) ELISA Kit

XY-EL-H0872c 人XIV型胶原 (COL14)ELISA试剂盒 Human COL14 (Collagen Type XIV) ELISA Kit

XY-EL-H0873c 人III型胶原交联羧基端肽(CTX-III)ELISA试剂盒 Human CTX-III (Cross Linked C-telopeptide of Type III Collagen) ELISA Kit

XY-EL-H0874c 人EJ抗体/抗甘氨酰tRNA合成酶抗体(EJ/GlyRS)ELISA试剂盒   Human EJ/GlyRS (Anti-EJ-Antibody) ELISA Kit