英文名称 CDK2
中文名称 周期素依赖性激酶2抗体
别 名 Cdc2 related protein kinase; cdc2-related protein kinase; Cdk 2; CDK2_HUMAN; Cell devision kinase 2; Cell division kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2).
说 明 书 0.1ml 0.2ml
研究领域 肿瘤 细胞生物 染色质和核信号 信号转导 细胞周期蛋白 激酶和磷酸酶
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 周期素依赖性激酶2抗体(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 33kDa
细胞定位 细胞核 细胞浆
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human CDK2
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
周期素依赖性激酶2抗体产品介绍 background:
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008].
Function:
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization.
周期素依赖性激酶2抗体
hz-K1505 rTaq DNA Polymerase 重组Taq DNA 聚合酶
hz-K1506 rTaq DNA Polymerase 重组Taq DNA 聚合酶
1207-10/50/100 动物RNAout(快速动物总RNA提取试剂)
3080-10/50 植物RNAout(植物RNA提取专用试剂)
3071-10/50 血液RNAout(血液总RNA提取试剂)
3073-10/50 病毒RNAout(病毒RNA提取试剂)
51102-10/100 细菌RNA提取试剂
1206-10/25/50 RNAin RNAin(非冻型RNA样品保存液)
R1100-20 TRIquick 总RNA提取试剂
R1200 TRIquickLS 液体样品RNA提取试剂
R1300 RNA wait RNA高效保存液
R1400 RNA plant 植物RNA提取的专用试剂
3130 RNAon(即用型RNA变性上样液)
3140 RNAsharp(RNA电泳带锐化剂)
P1250 蛋白抽提试剂盒-I (实体组织和细胞蛋白抽提)
P1255 蛋白抽提试剂盒-II (液体样品蛋白抽提和回收)
P1200 核-胞浆蛋白制备试剂盒 (Nuclear-Cytosol Extraction Kit)
hz-60 伊红 Y(Eosin Y Sodium)
hz-0001 Peroxidase (POD) Horseradish 辣根过氧化物酶
hz-0061 Trypan Blue 台盼蓝染色液
hz-0062 Trypan Blue 台盼蓝
hz-0063 Toluidine Blue O 甲苯胺蓝
hz-0064 X-gal
hz-0072 Ponceau S 丽春红S
hz-0028 FITC 异硫氢酸荧光素
hz-0067 p-Nitrophenyl Phosphate Disodium,pNPP 对硝基苯磷酸二钠
hz-0068 MTT 噻唑兰
hz-0069 OPD(o-Phenylenediamine) 邻苯二胺
hz-0083 ThzED 四甲基乙二胺
hz-0084 Activiated Biotin 活化生物素
PH-7 Rabbit IgG Sepharose 4B 兔IgG亲和层析柱
PH-8 Mouse IgG Sepharose 4B 小鼠IgG亲和层析柱
PH-5 Protein G-Sepharose 4B 重组蛋白G亲和层析柱
PH-6 rec Protein A-Sepharose FF 重组蛋白A亲和层析柱
周期素依赖性激酶2抗体 多少钱?
CST抗体/抗原的应用资料有吗?
CST抗体/抗原货号hz-10726R报价含票含运吗?
CST货号hz-10726R货期是多久?
沪震生物周期素依赖性激酶2抗体 可以申请试用吗?
沪震生物CST抗体/抗原售后政策是什么?
周期素依赖性激酶2抗体 有现货吗?