获取电话英文名称 beta subunit Cholera Toxin
中文名称 霍乱肠毒素β链抗体
别 名 beta subunit Cholera Toxin; Cholera enterotoxin B chain; Cholera enterotoxin beta chain; Cholera enterotoxin gamma chain; Cholera enterotoxin subunit B; Cholera toxin B protein; Choleragenoid; CTX B; CTXB; TOX B; TOXB; VC1456; CHTB_VIBCH.
说 明 书 0.1ml 0.2ml
研究领域 细菌及病毒
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Cholera Toxin
产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 霍乱肠毒素β链抗体（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 12kDa
细胞定位 细胞膜 分泌型蛋白
性 状 Lyophilized or Liquid
浓 度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from CTXB/beta subunit Cholera Toxin
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
霍乱肠毒素β链抗体产品介绍 background:
The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.
Function:
The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
霍乱肠毒素β链抗体
hz-0082 mouse serum 封闭用正常小鼠血清（原液）
hz-0007 Antibody Diluent 抗体稀释液
hz-0017 Citrate buffer(1M pH6.0) 抗原热修复试剂(100×浓缩液)
hz-0022 Poly-L-Lysine Solution(10×) 多聚赖氨酸(10×防脱片剂)
hz-0022-1 Poly-L-Lysine 多聚赖氨酸(防脱片剂)
hz-0025 poly-L-lysine-Prep Slides 多聚赖氨酸防脱磨砂玻片（防脱片载玻片）
hz-0025-1 Glass slides 载玻片
hz-0030 防脱片液(硫酸铬钾-明胶黏片剂粉剂)
hz-0021 HE Staining Kit 苏木素-伊红染色试剂盒
hz-0070 Citrate buffer powders(0.01M pH6.0) packages 柠檬酸盐缓冲液(抗原热修复试剂)（0.01M pH6.0）
hz-0071 Citrate buffer(1M pH6.0), Concentrate 柠檬酸盐缓冲液 （1M pH6.0）
hz-0073 Neutral balsam (Mounting Medium) 中性树胶封片剂
hz-0079 PBS (0.01M pH7.4) packages 磷酸盐缓冲液(0.01M pH7.4)（干粉包）
hz-0080 Cover Glass 盖玻片
hz-0081 slides box 塑料切片盒
hz-0089 Triton X-100 聚乙二醇辛基苯基醚
hz-0085 Tris 三羟甲基氨基甲烷
hz-0086 Tween 20 吐温20
hz-0013 WIP(Tissue and Cell lysis Reagent) WIP组织细胞裂解液
hz-0087 Staining rack(stainless steel) 不锈钢染色架
hz-0002 Red Blood Cell Lysing Buffer 红细胞裂解液
hz-0018-30 BCA kit BCA蛋白浓度测定试剂盒
hz-0018-120 BCA(bicinchoninic acid) BCA蛋白浓度测定试剂盒
hz-0019（暂无现货） Bradford Reagent Bradford 蛋白浓度测定试剂盒（暂无现货）
hz-0046 Acrylamide 丙烯酰胺
hz-0046-1 30％Acrylamide 30%丙烯酰胺
hz-0047 SDS-PAGE kit SDS-PAGE凝胶制备试剂盒
hz-0050 Tris-Glycine SDS Running Buffer Tris-甘氨酸蛋白电泳缓冲液(粉剂)
hz-0045 Sample Loading Buffer(5×) 上样缓冲液（5×）
hz-0088 SDS-PAGE Protein Loading Buffer-non reduced form SDS-PAGE 蛋白上样缓冲液-非还原型
P1300-70/250 Commassie Blue Fast Staining Solution 考马斯亮蓝快速染色液
