找不到产品?留下需求帮您找
一键询价牛ELISA试剂盒实验原理
本试剂盒应用双抗体夹心法测定标本中牛水平。用纯化的牛抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入牛,再与HRP标记的牛抗体结合,形成抗体-抗原-酶标抗体复合物,经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的牛呈正相关。用酶标仪在450nm波长下测定吸光度(OD值),通过标准曲线计算样品中牛浓度。
试剂盒组成:
1 | 30倍浓缩洗涤液 | 20ml×1瓶 | 7 | 终止液 6ml×1瓶 |
2 | 酶标试剂 | 6ml×1瓶 | 8 | 标准品(240 ng/L) 0.5ml×1瓶 |
3 | 酶标包被板 | 12孔×8条 | 9 | 标准品稀释液 1.5ml×1瓶 |
4 | 样品稀释液 | 6ml×1瓶 | 10 | 说明书 1份 |
5 | 显色剂A液 | 6ml×1瓶 | 11 | 封板膜 2张 |
6 | 显色剂B液 | 6ml×1/瓶 | 12 | 密封袋 1个 |
牛ELISA试剂盒ELISA试剂盒具有以下优点:
1)快速: 仅需传统夹心法ELISA 1/3的操作时间,1小时即可完成整个试验流程;
2)简单: 只需1个洗涤步骤;
3)灵活: 可单独或同时检测总蛋白及磷酸化蛋白,可在同一板上检测不同的靶蛋白;
4)灵敏: 达到甚至超过行业标准,且结果一致性良好;
5)兼容: 常规比色法检测,实验室常规酶标仪读数;
温馨提醒:ELISA加样时的防错小经验
(1)用一张写满字的纸,字越多越好,比如报纸,垫在下面,这样,加过标本的小孔看过去下面的字会变小,没加的字正常,非常容易区分。
(2)可以利用液面反光与没有加的孔加以区别
(3)在标本加完后,再把加样枪全压下,使液面产生小气泡,也可以加以区分
牛ELISA试剂盒公司热卖试剂盒:
HZB781Hu 人磷脂爬行酶1(PLSCR1)ELISA试剂盒 ELISA Kit for Phospholipid Scramblase 1 (PLSCR1)
HZB785Hu 人脑啡肽酶(NEP)ELISA试剂盒 ELISA Kit for Neprilysin (NEP)
HZB792Hu 人S100钙结合蛋白A8(S100A8)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A8 (S100A8)
HZB793Hu 人世间S100钙结合蛋白A9(S100A9)ELISA试剂盒 ELISA Kit for S100 Calcium Binding Protein A9 (S100A9)
HZB795Hu 人血清淀粉样蛋白A2(SAA2)ELISA试剂盒 ELISA Kit for Serum Amyloid A2 (SAA2)
HZB796Hu 人信号传导转录激活因子2(STAT2)ELISA试剂盒 ELISA Kit for Signal Transducer And Activator Of Transcription 2 (STAT2)
HZB801Hu 人激肽释放酶原(PK)ELISA试剂盒 ELISA Kit for Prekallikrein (PK)
HZB805Hu 人高分子量激肽原(HMWK)ELISA试剂盒 ELISA Kit for High Molecular Weight Kininogen (HMWK)
HZB808Hu 人粘蛋白7(MUC7)ELISA试剂盒 ELISA Kit for Mucin 7, Secreted (MUC7)
HZA680Hu 人棕榈酰化膜蛋白2(MPP2)ELISA试剂盒 ELISA Kit for Membrane Protein, Palmitoylated 2 (MPP2)
HZA681Hu 人乙醇脱氢酶1(ADH1)ELISA试剂盒 ELISA Kit for Alcohol Dehydrogenase 1 (ADH1)
HZB810Hu 人维生素D结合蛋白(DBP)ELISA试剂盒 ELISA Kit for Vitamin D Binding Protein (DBP)
HZB817Hu 人多功能蛋白聚糖(VS)ELISA试剂盒 ELISA Kit for Versican (VS)
HZB818Hu 人血管内皮生长因子受体1(VEGFR1)ELISA试剂盒 ELISA Kit for Vascular Endothelial Growth Factor Receptor 1 (VEGFR1)
HZB819Hu 人再生胰岛衍生蛋白4(REG4)ELISA试剂盒 ELISA Kit for Regenerating Islet Derived Protein 4 (REG4)
HZB822Hu 人角蛋白17(KRT17)ELISA试剂盒 ELISA Kit for Keratin 17 (KRT17)
HZB827Hu 人轴突生长诱向因子1(Ntn1)ELISA试剂盒 ELISA Kit for Netrin 1 (Ntn1)
HZB835Hu 人轴突生长诱向因子4(Ntn4)ELISA试剂盒 ELISA Kit for Netrin 4 (Ntn4)
HZB967Hu 人载脂蛋白A4(APOA4)ELISA试剂盒 ELISA Kit for Apolipoprotein A4 (APOA4)
HZB829Hu 人解整合素金属蛋白酶9(ADAM9)ELISA试剂盒 ELISA Kit for A Disintegrin And Metalloprotease 9 (ADAM9)
HZB830Hu 人组织转谷氨酰胺酶(TGM2)ELISA试剂盒 ELISA Kit for Transglutaminase 2, Tissue (TGM2)
HZB831Hu 人音猬因子(SHH)ELISA试剂盒 ELISA Kit for Hedgehog Homolog, Sonic (SHH)
HZA111Hu 人白介素12(IL12)ELISA试剂盒 ELISA Kit for Interleukin 12 (IL12)
HZB833Hu 人补体因子D(CFD)ELISA试剂盒 ELISA Kit for Complement Factor D (CFD)
HZB834Hu 人凝血因子Ⅺ(F11)ELISA试剂盒 ELISA Kit for Coagulation Factor XI (F11)
HZB839Hu 人纽蛋白(VCL)ELISA试剂盒 ELISA Kit for Vinculin (VCL)
HZB848Hu 人核因子κB抑制因子α(IκBα)ELISA试剂盒 ELISA Kit for Inhibitory Subunit Of NF Kappa B Alpha (IkBa)
HZB850Hu 人β-1,4-半乳糖转移酶1(β4GALT1)ELISA试剂盒 ELISA Kit for Beta-1,4-Galactosyltransferase 1 (b4GALT1)