人基质金属蛋白酶抑制因子4(TIMP-4)检测试剂盒实验操作常见问题分析
异常 | 结果描述 | 原因分析 | 对策 |
白板 | 显色步骤结束后,酶标板所有孔均无颜色。阳性对照不显色。 | 试剂已过效期,或不同试剂盒组分混用 | 检查试剂的组分及批号,确认未过期以及所有组分均属对应的试剂盒。不同试剂盒或不同批号的试剂不能混用。 |
错加、漏加试剂底物、显色剂 A或B | 严格按说明书的步骤操作,加完试剂后可观察一下液面高度是否一致,以减少漏加现象。 | ||
洗板及加样过程中,酶标受污染失活失去催化显色剂显色的能力 | 确认盛酶标的容器不含酶抑制剂如叠氮钠等,确认配制洗液的容器已洗干净。 | ||
终止液误作洗涤液稀释或当底物缓冲液配制 | 每次配人基质金属蛋白酶抑制因子4(TIMP-4)检测试剂盒制时都应看清标签标明物质 | ||
蒸馏水有问题 | 确认配制洗液的纯化水达到要求且未污染,与好蒸馏水比较。 |
标本及采集、贮运因素:严重溶血 ,以 HRP 为标记的ELISA 测定中,残留在孔内的血红蛋白具有过氧化物酶样活性,催化底物显色造成假阳性; 混有红细胞的血清 易沉淀或附着在聚乙烯孔内不易洗净;如有 细菌污染 ,菌体中可能含有内源性HRP,也会产生假阳性反应; 标本凝固不全 ,有时为了争取时间快速检测,常在血液还未开始凝固时即强行离心分离血清,使血清中仍残留部分纤维蛋白原,在ELISA测定过程中可以形成肉眼可见的纤维蛋白块,易造成假阳性结果; 采血试管洗涤不彻底 、反复使用易交叉污染; 塑料试管能吸附抗原物质 ,样本久置在塑料管内会使样本内抗原含量下降造成假阴性。
人基质金属蛋白酶抑制因子4(TIMP-4)检测试剂盒其它相关试剂盒:
xyD612Hu人蛋白酪氨酸磷酸酶样蛋白A(PTPLA)ELISA试剂盒ELISA Kit for Protein Tyrosine Phosphatase Like Protein A (PTPLA)
xyD589Hu人非受体型蛋白酪氨酸磷酸酶6(PTPN6)ELISA试剂盒ELISA Kit for Protein Tyrosine Phosphatase, Non ReHZptor Type 6 (PTPN6)
xyD951Hu人维甲酸受体β(RARβ)ELISA试剂盒ELISA Kit for Retinoic Acid ReHZptor Beta (RARb)
xyD952Hu人维甲酸受体γ(RARγ)ELISA试剂盒ELISA Kit for Retinoic Acid ReHZptor Gamma (RARg)
xyD965Hu人肝受体同源物1(LRH1)ELISA试剂盒ELISA Kit for Liver ReHZptor Homolog 1 (LRH1)
xyE389Hu人囊泡单胺转运蛋白2(VMAT2)ELISA试剂盒ELISA Kit for Vesicular Monoamine Transporter 2 (VMAT2)
xyE690Hu人N-酰基乙醇胺酸酰化酶(NAAA)ELISA试剂盒ELISA Kit for N-Acylethanolamine Acid Amidase (NAAA)
xyF010Hu人真核翻译起始因子4A2(EIF4A2)ELISA试剂盒ELISA Kit for Eukaryotic Translation Initiation Factor 4A2 (EIF4A2)
xyF723Hu人DNA指导聚合酶γ1(POLγ1)ELISA试剂盒ELISA Kit for Polymerase DNA Directed Gamma 1 (POLg1)
xyG095Hu人磷脂酰肌醇人基质金属蛋白酶抑制因子4(TIMP-4)检测试剂盒结合网格蛋白装配蛋白(PICALM)ELISA试剂盒ELISA Kit for Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM)
xyG202Hu人氧化应激诱导生长抑制因子1(OSGIN1)ELISA试剂盒ELISA Kit for Oxidative Stress InduHZd Growth Inhibitor 1 (OSGIN1)
xyG347Hu人B-Raf原癌基因丝氨酸/苏氨酸蛋白激酶(BRAF)ELISA试剂盒ELISA Kit for B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)
xyG456Hu人内质网氨肽酶2(ERAP2)ELISA试剂盒ELISA Kit for Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)
xyG782Hu人甲状腺转录因子1(TITF1)ELISA试剂盒ELISA Kit for Thyroid Transcription Factor 1 (TITF1)
xyH009Hu人肿瘤蛋白p53结合蛋白1(TP53BP1)ELISA试剂盒ELISA Kit for Tumor Protein p53 Binding Protein 1 (TP53BP1)
xyH069Hu人突触囊泡蛋白样蛋白2(SYTL2)ELISA试剂盒ELISA Kit for Synaptotagmin Like Protein 2 (SYTL2)
xyH163Hu人NAP关联蛋白(SNAPAP)ELISA试剂盒ELISA Kit for SNAP Associated Protein (SNAPAP)
xyH343Hu人副肿瘤抗原MA2(PNMA2)ELISA试剂盒ELISA Kit for Paraneoplastic Antigen MA2 (PNMA2)
xyH838Hu人异柠檬酸脱氢酶2(IDH2)ELISA试剂盒ELISA Kit for Isocitrate Dehydrogenase 2, mitochondrial (IDH2)
xyH923Hu人亨廷顿关联蛋白1(HAP1)ELISA试剂盒ELISA Kit for Huntingtin Associated Protein 1 (HAP1)
xyJ014Hu人G蛋白α抑制活性多肽3(GNαI3)ELISA试剂盒ELISA Kit for G Protein Alpha Inhibiting Activity Polypeptide 3 (GNaI3)
xyJ081Hu人葡萄糖-6-磷酸酶催化亚基(G6PC)ELISA试剂盒ELISA Kit for Glucose-6-Phosphatase, Catalytic (G6PC)
xyJ095Hu人脆性X智力迟钝蛋白1(FMR1)ELISA试剂盒ELISA Kit for Fragile X Mental Retardation 1 (FMR1)
xyK072Hu人Zeste同源物增强子1(EZH1)ELISA试剂盒ELISA Kit for EnhanHZr Of Zeste Homolog 1 (EZH1)
xyL504Hu人ER脂质Raft关联蛋白2(ERLIN2)ELISA试剂盒ELISA Kit for ER Lipid Raft Associated Protein 2 (ERLIN2)
xyQ214Hu人肿瘤关联糖蛋白72(TAG72)ELISA试剂盒ELISA Kit for Tumor Associated Glycoprotein 72 (TAG72)
xyL503Hu人ER脂质Raft关联蛋白1(ERLIN1)ELISA试剂盒ELISA Kit for ER Lipid Raft Associated Protein 1 (ERLIN1)
xyQ374Hu人地塞米松诱导蛋白(DEXI)ELISA试剂盒ELISA Kit for Dexamethasone InduHZd Protein (DEXI)