人基质金属蛋白酶抑制因子3(TIMP-3)ELISA试剂盒

人基质金属蛋白酶抑制因子3(TIMP-3)ELISA试剂盒试验所需自备物品:

1.  酶标仪（450nm波长滤光片）

2.  高精度移液器，EP管及一次性吸头：0.5-10μl, 2-20μl, 20-200μl, 200-1000μl

3.  37℃恒温箱, 双蒸水或去离子水

4.  吸水纸

检测前准备工作:

1. 请提前20分钟从冰箱中取出人基质金属蛋白酶抑制因子3(TIMP-3)ELISA试剂盒，平衡至室温。

2. 将浓缩洗涤液用双蒸水稀释（1:25）。未用完的放回4℃。从冰箱中取出的浓缩洗涤液可能有结晶，属于正常现象，可用40℃水浴微加热使结晶完全溶解后再配制洗涤液。（加热温度不要超过50℃）使用时洗涤液应为室温。

3.  标准品: 加入标准品&标本稀释液1.0ml至冻干标准品中，静置10分钟，待其充分溶解后，轻轻混匀（浓度为5000 pg/ml）。然后根据需要进行倍比稀释（注：不要直接在板中进行倍比稀释）。建议配制成以下浓度： 2500、1250、625、312.5、156.2、78.1、39.05、0 pg/ml。样品稀释液直接作为空白孔 0 pg/ml。如配制2500pg/ml标准品：取0.5ml （不要少于0.5ml ）5000pg/ml的上述标准品加入含有0.5ml样品稀释液的EP管中，混匀即可，其余浓度以此类推。人基质金属蛋白酶抑制因子3(TIMP-3)ELISA试剂盒

4. 生物素化抗体工作液：实验前计算当次实验所需用量（以100μl/孔计），实际配制时应多配制100-200μl。使用前15分钟，以生物素化抗体稀释液稀释浓缩生物素化抗体（1:100）成工作浓度。当日使用。

5. 酶结合物工作液：实验前计算当次实验所需用量（以100μl/孔计），实际配制时应多配制  100-200μl。使用前15分钟，以生物素化抗体稀释液稀释浓缩生物素化抗体（1:100）成工作浓度。当日使用。

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