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氧核糖核酸酶Ⅱ 来源于猪脾(纯化)
≥12,000 units/mg protein
Product Name: Deoxyribonuclease II from porcine spleen(Purified)
| CAS号: | 9025-64-3 |
| EINECS编号: | 232-801-8 |
| MDL号: | MFCD00130919 |
| 介绍: | Deoxyribonuclease II from porcine spleen has a molecular weight of 38 kDa. The enzyme is a glycoprotein endonuclease with dimeric structure. Optimum pH range is 4.5-5.0 at ionic strength 0.15 M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II. |
| 用途: | DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from bovine spleen has been used in a study that conducted a partial purification of deoxyribonucleases from eggs and liver of Xenopus laevis. Deoxyribonuclease II from bovine spleen has also been used in a study to investigate nucleic acid and protein synthesis of splenic lymphocytes. |
| 贮存: | 储存温度 -20°C |
| 生化和生理学机理: | The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass. |
氧核糖核酸酶Ⅱ 来源于猪脾(纯化)
生化试剂 氧核糖核酸酶Ⅱ 来源于猪脾(纯化)由上海信裕生物科技有限公司为您提供，如想了解更多关于常用生化试剂 氧核糖核酸酶Ⅱ 来源于猪脾(纯化)的报价、规格、厂家等信息，欢迎来电或留言咨询。除供应氧核糖核酸酶Ⅱ 来源于猪脾(纯化)外，上海信裕生物科技有限公司还可为您提供：
松脂醇二葡萄糖苷、
人嗜铬蛋白A(CgA)ELISA试剂盒 、
人生长调节致癌基因α/黑素瘤生长刺激因子(GROα/CXCL1/MGSA)ELISA试剂盒
