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核酸酶S1 来源于米曲霉

核酸酶S1 来源于米曲霉

价格: 1

品牌:Sigma-Aldrich

供货周期: 现货

货号:N128634

规格:AR级别,99%

N128634

核酸酶S1 来源于米曲霉

≥100,000-500,000 units/ml

Product Name: Nuclease S1 from Aspergillus oryzae

CAS号:37288-25-8
MDL号:MFCD00131010
介绍:Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.
用途:Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
贮存:储存温度 -20°C
生化和生理学机理:Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.

核酸酶S1 来源于米曲霉





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