用途:胆汁液态培养基用于分子生物学中的培养
原理:蛋白胨提供碳源和氮源;葡萄糖提供能源;琼脂是培养基的凝固剂。
用法:称取本品65.0g,加热搅拌溶解于1000ml蒸馏水中121℃高压灭菌15分钟,冷却至50℃左右时,加入过滤除菌的氯霉素50mg,混匀,倾入无菌平皿。
【注意事项】:由于液体培养基不易长期保管,现在均改制成粉末。
培养基有天然、人工两种胆汁液态培养基一般实验室使用的是RPMI-1640粉末培养基,以双蒸或三蒸水配制。NaHCO3(或HCl)调pH至7.2~7.4,若pH值超过7.6或远低于6.8,则大多数细胞不能生长。RPMI-1640不耐高压灭菌,使用前需经灭菌0.22μm孔径滤膜滤过处理。
胆汁液态培养基的保存
1)干粉,买了后一定要保存在4度。有人保存在-20度我认为没有必要。但4度是非常必要的。保证期是有提示的。
2)配好的无血清培养基一定保存在4度,保存不要超过3个月。用时根据提示要加入谷胺酰胺。
3)加入血清的培养基,最好保存在4度不要超过1个月,从时间太长,活性下降。当然稍微长点也不一定出问题。但是根据细胞而定;如果原代培养,最好新鲜,如果是肿瘤细胞,或仅维持传代就不太要紧。但是原则是越新越好。
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A-01146 ?anti-AQP1(aquaporin Protein 1)
A-01147 ?Anti-AQP2(aquaporin Protein-2)
A-01148 ?Anti-AQP3(aquaporin 3)
?Anti-AQP4(aquaporin Protein-4)
A-01150 ?Anti-AQP5(aquaporin Protein-5)
A-01151 ?Anti-AQP9(aquaporin Protein -9)
A-01152 ?Anti-AR(Androgen Receptor)
A-01153 ?Anti-phospho-AR(pSer580)(androgen receptor phospho-Ser580)
A-01154 ?Anti-AKR1B1/ADR(aldose reductase)
A-01155 ?Anti-ARC (ARG3.1 Activity-regulated cytoskeleton-associated protein)
A-01156 ?Anti-ARF6 (ADP-ribosylation factor 6)
A-01157 ?Anti-Arfaptin 1(ADP-ribosylation factor interacting protein 1 )
A-01158 ?Anti-ARFIP2/arfaptin 2(ADP-ribosylation factor interacting protein 2 )
A-01159 ?Anti-Aromatase (Cytochrome p450 19A1; Estrogen synthetase)
A-01160 ?Anti-Aromatase (Cytochrome p450 19A1; Estrogen synthetase)
A-01161 ?Anti-ARIP2a(Activin receptor 2A)
A-01162 ?Anti-β-arrestin 1(beta-arrestin 1)
A-01163 ?Anti-β-arrestin 2(beta-arrestin 2)
A-01164 ?Anti-ARIP2B(Activin receptor 2B)
A-01165 ?Anti-ART(Artemin)
A-01166 ?Anti-ADRα1(α1-adrenergic receptor)
A-01167 ?Anti-ASCL1/MASH1(Achaete-scute homolog 1)
A-01168 ?Anti-ASPP1(apoptosis stimulating protein of P53 1)
A-01169 ?Anti-ASPP2(apoptosis stimulating protein of P53 2)
A-01170 ?Anti-AT/AGT(Angiotenogen)
A-01171 ?Anti-Ang I/AT1(Angiotensin I)
A-01172 ?Anti-AT1R/AGTR1(Angiotensin II Type 1 Receptor)
A-01173 ?Anti-AT1R(Angiotensin II Type 1 Receptor)
A-01174 ?Anti-ATF1 (Activating Transcription Factor1)
A-01175 ?Anti-ATF2 (Activating Transcription Factor2)
A-01176 ?Anti-phospho-ATF2(pSer490/pSer498)
A-01177 ?Anti-ATF3 (Activating Transcription Factor3)
A-01178 ?Anti-ATG7(Autophagy-related protein 7)
A-01179 ?Anti-ATF4(activating transcription factor 4)
A-01180 ?Anti-ATM(ataxia telangiectasia mutated)