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2-硝基苯基-β-D-吡喃半乳糖苷
Product Name: 2-Nitrophenyl β-D-galactopyranoside
别名: 2-硝基苯基-β-D-吡喃半乳糖苷(ONPG) ;邻硝基苯-β-D-吡喃半乳糖苷
Cas号: 369-07-3
分子式: C12H15O8N
分子量: 301.25
EINECS编号: 206-716-1
MDL号: MFCD00063255
N109040-1g 99%，非动物源
N109040-25g 99%，非动物源
N109040-5g 99%，非动物源
2-硝基苯基-β-D-吡喃半乳糖苷介绍: 溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。
用途: 用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。
包装: N109040-25gglass btl
N109040-1gglass btl
N109040-5gpoly btl
熔点: 195℃
特定比重: 1.599
比旋光度: -68.8 ° (C=1, H2O)
贮存: -20℃充氩保存
2-硝基苯基-β-D-吡喃半乳糖苷生化和生理学机理: β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
