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产品名称： 猴促肾上皮质激素释放激素(CRH)ELISA kit
英文名称： Monkey corticoliberin,CRH ELISA kit
货号： CSB-EL005963MK
规格： 96T
种属： Monkey
待测物名称： corticotropin releasing hormone
缩写： CRH
反应时间： 1-5h
所需样本体积： 50-100ul
检测波长： 450 nm
猴促肾上皮质激素释放激素(CRH)ELISA kit
用途： For research use only. Not for diagnostic use.
猴促肾上皮质激素释放激素(CRH)ELISA kit
原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
猴促肾上皮质激素释放激素(CRH)ELISA kit
特异性： This assay has high sensitivity and excellent specificity for detection of Monkey CRH. No significant cross-reactivity or interference between Monkey CRH and analogues was observed.
猴促肾上皮质激素释放激素(CRH)ELISA kit
精密度： Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
猴促肾上皮质激素释放激素(CRH)ELISA kit
样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
猴促肾上皮质激素释放激素(CRH)ELISA kit
检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
猴促肾上皮质激素释放激素(CRH)ELISA kit
结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CRH concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
猴促肾上皮质激素释放激素(CRH)ELISA kit
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猴促肾上皮质激素释放激素(CRH)ELISA kit
抗体
产品名称 货号 规格
Rabbit anti-human corticotropin releasing hormone polyclonal Antibody CSB-PA005963GA01HU 150ul
猴促肾上皮质激素释放激素(CRH)ELISA kit
蛋白
产品名称 货号 规格 蛋白来源
Recombinant Bacillus subtilis HPr-like protein crh(crh) CSB-EP519170BRJ 1mg E.coli
Recombinant Bacillus subtilis HPr-like protein crh(crh) CSB-YP519170BRJ 1mg Yeast
Recombinant Xenopus laevis Corticoliberin(crh) CSB-EP005963XBE 1mg E.coli
Recombinant Xenopus laevis Corticoliberin(crh) CSB-YP005963XBE 1mg Yeast
Recombinant Rat Corticoliberin(Crh) CSB-EP005963RA 1mg E.coli
Recombinant Rat Corticoliberin(Crh) CSB-YP005963RA 1mg Yeast
Recombinant Sheep Corticoliberin(CRH) CSB-EP005963SH 1mg E.coli
Recombinant Sheep Corticoliberin(CRH) CSB-YP005963SH 1mg Yeast
Recombinant Human Corticoliberin(CRH) CSB-EP005963HU 1mg E.coli
Recombinant Human Corticoliberin(CRH) CSB-YP005963HU 1mg Yeast
Recombinant Pig Corticoliberin(CRH) CSB-EP005963PI 1mg E.coli
Recombinant Pig Corticoliberin(CRH) CSB-YP005963PI 1mg Yeast
Recombinant Dog Corticoliberin(CRH) CSB-EP005963DO 1mg E.coli
Recombinant Dog Corticoliberin(CRH) CSB-YP005963DO 1mg Yeast