abbelight
3D超分辨成像系统
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abbelight是一款模块化的多功能的单分子定位显微镜(SMLM)系统,它独有的DASEY技术能够极大的提高定位精度的同时,还保持在较小的尺寸。该设备具有高度灵活性,能够搭载在绝大多数的倒置显微镜上,并且仅仅需要使用一个C-mount(CCD或CMOS所连接的部位)接口,即可将您的倒置显微镜直接升级为超分辨显微镜。并且改造过程不会破坏原有显微镜系统的光路和功能,不会与其它的显微镜改造相冲突。
本设备既在配置上的选择也十分灵活。它既可以作为显微镜的一个升级配件来改造您的显微镜,也拥有完整的超分辨系统。让用户在获得专业的图像质量的同时,享受到最为经济合理的超分辨升级方案。
![image002.png image002.png](https://img1.17img.cn/17img/images/201907/uepic/f695a990-5683-4a2b-9911-24419e9fbba3.jpg)
• 成像模式:PLAM、STORM、smFRET、SPT • 光源模式:Epi、TIRF、HILO • 25 nm的XY轴分辨率 • 200 × 200 μm2的超大视野 • 全自动化控制 • 无需高功率激光光源 • 可升级SAFe 360
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大视野3D超分辨模块 |
• 具有SAFe 180的所有功能 • 15 nm的XYZ轴分辨率 • 一次可同时采集1.2 μm深度图像信息 • 最高图像深度:10 μm • 实时漂移矫正 • 最高四色同时成像 • 活细胞成像模式 | ![image004.png image004.png](https://img1.17img.cn/17img/images/201907/uepic/4858ba95-0d93-495c-87d7-649e800dde88.jpg)
高精准3D超分辨显微成像模块 |
加装 | TIRF PALM STORM SPT
smFRET ...... | 兼容 | Confocal Spinning-Desk Widefield SIM STED
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Now We See......
配套试剂
Smart kit | Compatible dyes |
• 10 doses per box • 200 μL per dose • 30 sec prepartion • 2 months in a fridge • 2 weeks on sample
| • Phalloidin-AF ® 488, WGA-AF ® 488 • AF ® 532, CF ® 532, Cy3b • AF ® 555, CF ® 555, AF ® 568, CF ® 568, Cy5, MemBrite™ 568 • AF ® 647, CF ® 647, AF ® 680, CF ® 680, MemBrite™ 640
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发表文献列表
1. TADs are 3D structural units of higher-order chromosome organization in Drosophila, Science Advances 2018, Article reference DOI: 10.1126/sciadv.aar8082
2. Multicolor localization microscopy by deep learning, ArXiv 2018, Article reference arXiv:1807.01637
3. Primary fibroblasts derived from sporadic amyotrophic lateral sclerosis patients do not show ALS cytological lesions, Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration 2018, Article reference DOI: 10.1080/21678421.2018.1431787
4. Quantitative mapping and minimization of super-resolution optical imaging artifacts, Nature Methods 2018, DOI: 10.1038/nmeth.4605
5. Combining 3D single molecule localization strategies for reproducible bioimaging, BioRxiv 2018, Article reference DOI: 10.1101/385799
6. Aberration-accounting calibration for 3D single-molecule localization microscopy, Optics Letters 2017, Article reference DOI: 10.1364/OL.43.000174
7. Podosome Force Generation Machinery: A Local Balance between Protrusion at the Core and Traction at the Ring, ACS Nano 2017, Article reference DOI: 10.1021/acsnano.7b00622
8. Failure of daptomycin to kill Staphylococcus aureus: impact of bacterial membrane fatty acid composition, Antimicrobial Agents and Chemotherapy 2018, Article reference DOI:10.1128/AAC.00023-18
9. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment, Neuron 2017, Article reference DOI: 10.1016/j.neuron.2017.12.039
10. Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy, Scientific Reports 2017, DOI: 10.1038/s41598-017-15433-2
创新点:
abbelight既可以作为显微镜的一个配件将您的显微镜升级至超分辨,也拥有完整的超分辨系统;独有的DAISY技术,使XYZ轴精度达到15nm,超精准定位;拥有200μm x 200μm的超大视野,最高可实现四色同时成像!
3D超分辨成像系统